Effect of Ethylene Glycol on the Expression of Exogenous Genes in Vivo

doi:10.13560/j.cnki.biotech.bull.1985.2018-0675

Abstract

Abstract: This work aims to explore the effect of ethylene glycol on the expression of exogenous genes in mouse liver in hydrodynamics-based transgene. The pEGFP-C1 plasmid solution containing different concentrations of ethylene glycol was injected into the tail vein of mice by hydrodynamics-based transgene. The frozen slices of the right lobe of the mice liver were taken at 24 h,48 h and 72 h after the tail vein injection,and were observed with a fluorescence microscope at 488 nm excitation wavelength and the green fluorescent protein（GFP）expression was quantified. At the same time,hematoxylin staining was also carried out and the morphology of the mouse liver was observed. The positive cell rate of GFP at 24 h after transgenic tended to decrease with the increase of ethylene glycol concentration when the concentration of ethylene glycol ranged in 0.05-0.5 mol/L;while the positive cell rate of GFP at 72 h increased with the concentration of ethylene glycol,and was quite high at concentration of ethylene glycol of 0.5 mol/L. In conclusion,ethylene glycol may regulate the expression of exogenous genes in hydrodynamics-based transgene,and the expression peaks shift backwards.

Key words: mice, hydrodynamics-based transgene, pEGFP-C1, ethylene glycol

WANG Qi-wen, LI Pan, Pan Cui-yun, HAN Fen-xia. Effect of Ethylene Glycol on the Expression of Exogenous Genes in Vivo[J]. Biotechnology Bulletin, 2019, 35(4): 64-68.

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