Characteristic and Expression Analysis of ASR Gene from Banana

Abstract

Abstract: ASR gene was obtained from banana cDNA libraries in this study and ws named as MaASR. Phylogenetic analysis revealed MaASR was close relatives to Musaceae of Monocotyledons. Endogenous ABA content was determined in naturally ripened bananas and ethylenetreated bananas. In naturally ripened bananas, endogenous ABA content is relatively low at mature early(0-2 d), and increase maximum at 8 day, then drop rapidly. In ethylene- treated bananas, ABA content rise sharply peak at 3 days and then gradually decline. Real-time quantitative PCR analysis expression of MaASR in naturally ripened bananas and ethylene- treated bananas. In naturally ripened bananas, MaASR expression increased and peaked 2 days after harvest, then fell rapidly and remained low at 6-16 d. While in ethylene- treated bananas, MaASR expression levels do not show the dramatic change trend, the expression is overall low. The results showed that MaASR was responding to ethylene signal,but response intensity is low, its expression trend was negatively correlated with the content of ABA or signal strength.

Key words: Banana, ASR gene, Characteristic Expression analysis

Jia Caihong, Xu Biyu, Liu Juhua, Zhang Jianbin, Feng Renjun, Jin Zhiqiang. Characteristic and Expression Analysis of ASR Gene from Banana[J]. Biotechnology Bulletin, 2014, 0(1): 105-111.

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