Expression Analysis of Fusarium Wilt Resistance-Related Genes at the Flower Bud Differentiation Stage of Banana

doi:10.13560/j.cnki.biotech.bull.1985.2017-0071

Abstract

Abstract: A large number of differentially expressed genes were obtained from transcriptome analysis of ‘Brazilian’ and ‘Formosana’ bananas infected by Fusarium oxysporum f. sp. cubense race 4（Foc4）at the flower bud differentiation stage. The expression variations of 10 disease-resistance related genes from diseased ‘Brazilian’ and ‘Formosana’ banana roots were detected by real-time quantitative PCR（qPCR）. The results showed that these 10 genes expressed in both ‘Brazilian’ and ‘Formosana’ at flower bud differentiation，but the expression levels of these genes varied. Compared with the control of ‘Brazilian’ banana，the expression levels of the nine genes（laccase-4（B），periplasmic beta-glucosidase precursor，glutathione-S-transferase Cla47，class III peroxidase 136 precursor，peroxidase N，similar to T-phylloplanin，snakin-1，putative chitinase，and ellulose synthase catalytic subunit 12）up-regulated except gene laccase-4（A）. Among them，the expression of peroxidase N significantly up-regulated，and the relative expression was 1 682 times as much as in ‘Brazilian’. These results suggested that 10 differentially expressed genes may be involved in defense responses against Foc4 at the flower bud differentiation stage of ‘Formosana’，which lay a foundation for the further study on molecular mechanisms of ‘Formosana’ defense against fusarium wilt.

Key words: banana fusarium wilt, flower bud differentiation stage, disease-resistance related gene, qPCR

WANG Gui-hua, ZHANG Xin ,QI Yan-xiang ,WANG Yu-guang ,PENG Jun ,XIE Yi-xian. Expression Analysis of Fusarium Wilt Resistance-Related Genes at the Flower Bud Differentiation Stage of Banana[J]. Biotechnology Bulletin, 2017, 33(7): 83-88.

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