Cloning and Activity Analysis of Soybean GmERF5 Promoter

Abstract

Abstract: ERF transcription factor family is extensively involved in the biotic and abiotic responses. The GmERF5 promoter(1 924 bp) was isolated from soybean genome using PCR. Sequence analysis indicated that GmERF5P contains several stress-induced elements: G-box,GT-1, LTRE-1, W-box, TL1, DPBF, MYB, MYC and BIHD10S. GmERF5P was subcloned into pCAMBIA1301 vector to drive the GUS gene to express in tobacco leaves by Agrobacterium-mediated injection transformation. The activity of GmERF5P was analyzed using histochemistry staining and GUS fluorescence intensity analysis. The results indicated that GmERF5P activated expression of GUS report gene under normal growing conditions. The activity of GmERF5P was up-regulated after the treatments of drought and low temperature for 10 h.

Key words: Soybean, GmERF5P, GUS activity, Promoter, Stress-induced

Zhai Ying, Zhang Jun, Zhao Yan, Sun Tianguo, Yao Yanan, Yang Xiaojie. Cloning and Activity Analysis of Soybean GmERF5 Promoter[J]. Biotechnology Bulletin, 2014, 0(1): 100-104.

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