Abstract: To analyze the biodiversity of salt-tolerant Bacillus in the northeast of China, the temperature screening and gradient plate methods were used. 137 Bacillus strains were obtained, in which has 74 salt-tolerant Bacillus strains, 54% of total Bacillus strains. The optimum salt concentrations of these salt-tolerant Bacillus strains were all 1% and the range were 4%-14%. 16S rRNA sequences were amplified and analyzed for the determination of phylogenetic relationships. The 36 salt-tolerant Bacillus strains were different from each other in their 16S rRNA sequences or the salt tolerance, classified into 7 species of Bacillus genera. Among them, Bacillus thuringiensis was majority（14 strains, 38.9% of all, highest salt tolerance were 4%-9%NaCl）, second were Bacillus cereus（7, 19.4%, 4%-8%）and Bacillus subtilis（7, 19.4%, 8%-11%）, then Bacillus anthracis（4, 11.1%, 5%-7%）, Bacillus flexus（2, 5.6%, 9%-14%）, Bacillus sphaericus（1, 2.8%, 5%）and Bacillus aryabhattai（1, 2.8%, 6%）. Bacillus flexus and Bacillus subtilis were shown better salt tolerance. Cultural and morphology characteristics, biochemical indexes and phylogenetic analysis of 3 typical strains were given. Provide an effective data support for the discovery of salt-tolerant Bacillus.

Key words: Salt-tolerant, Bacillus, Isolation, Primary identification, 16S rRNA