Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (8): 262-271.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1574
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CHU Rui1,2(), LI Zhao-xuan1,2, ZHANG Xue-qing1,2, YANG Dong-ya1,2, CAO Hang-hang1,2, ZHANG Xue-yan1,2()
Received:
2023-01-02
Online:
2023-08-26
Published:
2023-09-05
Contact:
ZHANG Xue-yan
E-mail:churui0314@163.com;zhangxueyan123@sina.com
CHU Rui, LI Zhao-xuan, ZHANG Xue-qing, YANG Dong-ya, CAO Hang-hang, ZHANG Xue-yan. Screening and Identification of Antagonistic Bacillus spp. Against Cucumber Fusarium wilt and Its Biocontrol Effect[J]. Biotechnology Bulletin, 2023, 39(8): 262-271.
菌株Strain | 菌落直径Colony diameter/cm | 抑菌率Inhibition rate/% | 菌株Strain | 菌落直径Colony diameter/cm | 抑菌率Inhibition rate/% | |
---|---|---|---|---|---|---|
N1 | 2.30 | 72.18 | N26 | 3.03 | 60.84 | |
N2 | 2.40 | 70.63 | N27 | 3.17 | 58.78 | |
N3 | 2.40 | 70.63 | N28 | 2.79 | 58.48 | |
N4 | 2.42 | 70.38 | N29 | 2.80 | 58.33 | |
N5 | 2.43 | 70.12 | N30 | 2.84 | 57.58 | |
N6 | 2.47 | 69.60 | N31 | 2.84 | 57.58 | |
N7 | 2.50 | 69.09 | N32 | 3.28 | 56.98 | |
N8 | 2.58 | 68.81 | N33 | 2.88 | 56.82 | |
N9 | 2.53 | 68.57 | N34 | 2.93 | 55.92 | |
N10 | 2.53 | 68.57 | N35 | 2.95 | 55.62 | |
N11 | 2.53 | 68.57 | N36 | 3.85 | 48.22 | |
N12 | 2.55 | 68.32 | N37 | 4.11 | 44.26 | |
N13 | 2.55 | 68.32 | N38 | 4.16 | 43.48 | |
N14 | 2.60 | 67.54 | N39 | 4.69 | 35.24 | |
N15 | 2.63 | 67.03 | N40 | 4.27 | 31.76 | |
N16 | 2.65 | 66.77 | N41 | 4.38 | 29.65 | |
N17 | 2.68 | 66.25 | N42 | 5.02 | 18.18 | |
N18 | 2.73 | 65.48 | N43 | 5.40 | 11.23 | |
N19 | 2.77 | 64.97 | N44 | 5.50 | 9.42 | |
N20 | 2.80 | 64.45 | N45 | 5.80 | 3.99 | |
N21 | 2.80 | 64.45 | N46 | 5.80 | 3.99 | |
N22 | 2.85 | 63.68 | N47 | 5.82 | 3.68 | |
N23 | 2.87 | 63.42 | N48 | 5.88 | 2.48 | |
N24 | 2.88 | 63.16 | N49 | 7.10 | 1.95 | |
N25 | 2.97 | 61.88 | N50 | 6.97 | 0.00 |
Table 1 Inhibition of antagonist Bacillus strain against Fusarium oxysporum f. sp. cucumerinum(FOC)
菌株Strain | 菌落直径Colony diameter/cm | 抑菌率Inhibition rate/% | 菌株Strain | 菌落直径Colony diameter/cm | 抑菌率Inhibition rate/% | |
---|---|---|---|---|---|---|
N1 | 2.30 | 72.18 | N26 | 3.03 | 60.84 | |
N2 | 2.40 | 70.63 | N27 | 3.17 | 58.78 | |
N3 | 2.40 | 70.63 | N28 | 2.79 | 58.48 | |
N4 | 2.42 | 70.38 | N29 | 2.80 | 58.33 | |
N5 | 2.43 | 70.12 | N30 | 2.84 | 57.58 | |
N6 | 2.47 | 69.60 | N31 | 2.84 | 57.58 | |
N7 | 2.50 | 69.09 | N32 | 3.28 | 56.98 | |
N8 | 2.58 | 68.81 | N33 | 2.88 | 56.82 | |
N9 | 2.53 | 68.57 | N34 | 2.93 | 55.92 | |
N10 | 2.53 | 68.57 | N35 | 2.95 | 55.62 | |
N11 | 2.53 | 68.57 | N36 | 3.85 | 48.22 | |
N12 | 2.55 | 68.32 | N37 | 4.11 | 44.26 | |
N13 | 2.55 | 68.32 | N38 | 4.16 | 43.48 | |
N14 | 2.60 | 67.54 | N39 | 4.69 | 35.24 | |
N15 | 2.63 | 67.03 | N40 | 4.27 | 31.76 | |
N16 | 2.65 | 66.77 | N41 | 4.38 | 29.65 | |
N17 | 2.68 | 66.25 | N42 | 5.02 | 18.18 | |
N18 | 2.73 | 65.48 | N43 | 5.40 | 11.23 | |
N19 | 2.77 | 64.97 | N44 | 5.50 | 9.42 | |
N20 | 2.80 | 64.45 | N45 | 5.80 | 3.99 | |
N21 | 2.80 | 64.45 | N46 | 5.80 | 3.99 | |
N22 | 2.85 | 63.68 | N47 | 5.82 | 3.68 | |
N23 | 2.87 | 63.42 | N48 | 5.88 | 2.48 | |
N24 | 2.88 | 63.16 | N49 | 7.10 | 1.95 | |
N25 | 2.97 | 61.88 | N50 | 6.97 | 0.00 |
菌株 Strain | 菌落直径 Colony diameter/cm | 抑菌率 Inhibition rate/% |
---|---|---|
N1 | 2.08 | 77.71 |
N3 | 2.20 | 75.94 |
N6 | 2.28 | 74.76 |
N7 | 2.30 | 74.53 |
N2 | 2.32 | 74.29 |
N4 | 2.49 | 71.82 |
Table 2 Inhibition rate of Bacillus spp. re-screening on FOC
菌株 Strain | 菌落直径 Colony diameter/cm | 抑菌率 Inhibition rate/% |
---|---|---|
N1 | 2.08 | 77.71 |
N3 | 2.20 | 75.94 |
N6 | 2.28 | 74.76 |
N7 | 2.30 | 74.53 |
N2 | 2.32 | 74.29 |
N4 | 2.49 | 71.82 |
试验项目 Test project | 结果 Result | |||
---|---|---|---|---|
N1 | N3 | N6 | ||
甲基红(M-R)Methyl red | - | - | - | |
接触酶Catalase | - | - | - | |
氧化酶Oxidase | + | + | + | |
淀粉水解Amylum hydrolysis | - | - | - | |
纤维素酶Cellulase | - | - | - | |
明胶液化Gelatin experiment | + | - | + | |
乙酰甲基甲醇(V-P)Voges-Proskauer | - | - | - | |
葡萄糖氧化发酵Oxidation-fermentation of glucose | + | + | + |
Table 3 Physiological and biochemical results of N1, N3, and N6 strains
试验项目 Test project | 结果 Result | |||
---|---|---|---|---|
N1 | N3 | N6 | ||
甲基红(M-R)Methyl red | - | - | - | |
接触酶Catalase | - | - | - | |
氧化酶Oxidase | + | + | + | |
淀粉水解Amylum hydrolysis | - | - | - | |
纤维素酶Cellulase | - | - | - | |
明胶液化Gelatin experiment | + | - | + | |
乙酰甲基甲醇(V-P)Voges-Proskauer | - | - | - | |
葡萄糖氧化发酵Oxidation-fermentation of glucose | + | + | + |
试验项目 Test project | 结果 Result | ||
---|---|---|---|
N1 | N3 | N6 | |
铁载体Siderophore | + | + | + |
解钾 Releasing potassium | + | + | + |
溶磷Releasing phosphorus | - | - | - |
固氮Nitrogen-fixing ability | - | - | - |
蛋白酶 Protease | + | + | + |
几丁质酶Chitinase | + | + | + |
果胶酶Pectinase | + | + | + |
ACC脱氨酶活性ACC deaminase efficiency | - | - | - |
Table 4 Identification of disease resistance of N1, N3, and N6 strains and growth- promoting characteristics
试验项目 Test project | 结果 Result | ||
---|---|---|---|
N1 | N3 | N6 | |
铁载体Siderophore | + | + | + |
解钾 Releasing potassium | + | + | + |
溶磷Releasing phosphorus | - | - | - |
固氮Nitrogen-fixing ability | - | - | - |
蛋白酶 Protease | + | + | + |
几丁质酶Chitinase | + | + | + |
果胶酶Pectinase | + | + | + |
ACC脱氨酶活性ACC deaminase efficiency | - | - | - |
处理 Treatment | 接种尖镰孢菌后的时间Time after inoculation with FOC/d | |||||||
---|---|---|---|---|---|---|---|---|
10 | 14 | 18 | 22 | |||||
病情指数 Disease index | 防病效果 Control efficiency/% | 病情指数 Disease index | 防病效果 Control efficiency/% | 病情指数 Disease index | 防病效果 Control efficiency/% | 病情指数 Disease index | 防病效果 Control efficiency/% | |
CK2 | 12.50 | 33.33 | 69.44 | 86.11 | ||||
N1 | 9.72 | 22.22 | 8.33 | 75.00 | 22.22 | 68.00 | 50.00 | 41.93 |
N3 | 9.72 | 22.22 | 20.83 | 37.49 | 34.72 | 50.00 | 68.06 | 20.97 |
N6 | 5.56 | 55.56 | 8.33 | 75.00 | 12.50 | 82.00 | 29.17 | 66.13 |
Table 5 Disease-prevention effect of the strain on cucumber wilt
处理 Treatment | 接种尖镰孢菌后的时间Time after inoculation with FOC/d | |||||||
---|---|---|---|---|---|---|---|---|
10 | 14 | 18 | 22 | |||||
病情指数 Disease index | 防病效果 Control efficiency/% | 病情指数 Disease index | 防病效果 Control efficiency/% | 病情指数 Disease index | 防病效果 Control efficiency/% | 病情指数 Disease index | 防病效果 Control efficiency/% | |
CK2 | 12.50 | 33.33 | 69.44 | 86.11 | ||||
N1 | 9.72 | 22.22 | 8.33 | 75.00 | 22.22 | 68.00 | 50.00 | 41.93 |
N3 | 9.72 | 22.22 | 20.83 | 37.49 | 34.72 | 50.00 | 68.06 | 20.97 |
N6 | 5.56 | 55.56 | 8.33 | 75.00 | 12.50 | 82.00 | 29.17 | 66.13 |
处理 Treatment | 株高 Stem length/cm | 茎粗 Stem diameter/mm | 叶面积 Leaf area/cm2 | 叶绿素含量Chlorophyll content/SPAD | 地上部鲜重 Fresh weight of above ground/g | 地下部鲜重Fresh weight of below ground/g |
---|---|---|---|---|---|---|
CK1 | 17.66±0.73a | 3.9±0.11b | 32.65±0.87a | 39.50±0.72b | 5.55±0.13b | 1.67±0.23ab |
CK2 | 13.84±0.95b | 2.77±0.06c | 23.21±2.42b | 24.35±1.21c | 2.54±0.46c | 1.03±0.14b |
N1 | 15.68±0.52ab | 4.17±0.07ab | 35.80±2.79a | 45.85±1.30a | 7.15±0.4ab | 1.44±0.08ab |
N3 | 14.46±0.25b | 4.24±0.15a | 32.49±2.18a | 44.68±1.45a | 6.45±0.86b | 1.47±0.25ab |
N6 | 16.83±0.37a | 4.33±0.09a | 34.34±1.78a | 42.9±0.81ab | 8.39±0.45a | 1.76±0.19a |
Table 6 Growth-promoting effect of strains on cucumber seedlings
处理 Treatment | 株高 Stem length/cm | 茎粗 Stem diameter/mm | 叶面积 Leaf area/cm2 | 叶绿素含量Chlorophyll content/SPAD | 地上部鲜重 Fresh weight of above ground/g | 地下部鲜重Fresh weight of below ground/g |
---|---|---|---|---|---|---|
CK1 | 17.66±0.73a | 3.9±0.11b | 32.65±0.87a | 39.50±0.72b | 5.55±0.13b | 1.67±0.23ab |
CK2 | 13.84±0.95b | 2.77±0.06c | 23.21±2.42b | 24.35±1.21c | 2.54±0.46c | 1.03±0.14b |
N1 | 15.68±0.52ab | 4.17±0.07ab | 35.80±2.79a | 45.85±1.30a | 7.15±0.4ab | 1.44±0.08ab |
N3 | 14.46±0.25b | 4.24±0.15a | 32.49±2.18a | 44.68±1.45a | 6.45±0.86b | 1.47±0.25ab |
N6 | 16.83±0.37a | 4.33±0.09a | 34.34±1.78a | 42.9±0.81ab | 8.39±0.45a | 1.76±0.19a |
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