Expression of FtsZ Protein from Xanthomonas oryzae in Escherichia coli

Abstract

Abstract: It was to prepare FtsZ protein using techniques of modern molecular biology. The ftsZ gene was amplified from Xanthomonas oryzae by nested PCR, and recombinant plasmid pET-22b-ftsZ was constructed and transformed to E.coli BL21. The clony fragment was identificatified by PCR screening, Nde I/Xho I digestion and DNA sequencing, the positive clones were induced by IPTG for expression；the fusion protein was purified through Ni-NTA Resin, and identified by SDS-PAGE and Western blotting. Results showed that the recombinant plasmid pET-22b-ftsZ was constructed successfully, the FtsZ-6×His fusion protein was expressed in recombined E. coli BL21 induced by IPTG, and purified through Ni-NTA Resin by electrophoretic purity.

Key words: FtsZ protein, Xanthomonas oryzae , Escherichia coli , Genetic recombination

Chen Yang, Huang ,Yunhong, Li Suzhen, Long Zhonger. Expression of FtsZ Protein from Xanthomonas oryzae in Escherichia coli[J]. Biotechnology Bulletin, 2014, 0(11): 174-178.

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