Abstract: The morphology and molecular cytogenetic testing of 15 materials were conducted in F5 generation of perennial wheat hybrids obtained from octoploid Trititrigia and Thinopyrumintermedium hybridization. The results showed that most of the materials were contained in E and St chromosomes or chromosome fragments. Among them, eight intermediate types(Tritelytrigia type)had the parents’ traits, such as developed root system, lush plants, more tillers, and powerful stress resistance, and so on. But the number of chromosomes was remained instability, ranging from 42-56, and there were six materials with renewable trait. In addition, seven common wheat types that the number of chromosomes was between 41-43, they were no regeneration, but contained Thinopyrumintermediate chromosome or chromosome fragment. Those common wheat types had a large and multiflorous spike, disease resistance and other characteristics, which may be E or St chromosome substitution or translocation. These results indicated that the genes of perennial wheat that determined renewable, cold resistance and perennial characteristic were primarily in the part of E and St chromosomes.

Key words: Thinopyrum intermedium, Perennial wheat, Morphological characteristics, Molecular cytogenetics, Chromosomes