Cloning and Expression Pattern Analysis of Actin Gene Fragment from Elytrigia elongata

Abstract

Abstract: A pair of degenerate primers was designed based on the conserved sequences of the actin(ACT)genes from other plants. Total RNA was extracted from the roots of Elytrigia elongata to obtain an ACT gene fragment by reverse transcription polymerase chain reaction(RT-PCR). Results showed that the length of EeACT gene fragment was 598 bp encoding a 198 amino acids. The deduced amino acid sequence of EeACT shared over 90% amino acid homology compared with other plants ACT. No significant differences in expression level of EeACT were found between shoots and roots among low temperature, salt and drought treatments for 12 h, indicating that expression of EeACT is stable.

Key words: Elytrigia elongata, Actin gene, RT-PCR, Homo-cloning

Guo Qiang, Meng Lin, Mao Peichun, Tian Xiaoxia, Li shanshan, Zhang Lin. Cloning and Expression Pattern Analysis of Actin Gene Fragment from Elytrigia elongata[J]. Biotechnology Bulletin, 2014, 0(3): 73-78.

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Cloning and Expression Pattern Analysis of Actin Gene Fragment from Elytrigia elongata

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[1]	SUN Bao-zhen, QUAN Long-ping, KANG Hui, YAO Yu-xin, SHEN Tian, CHEN Wei-ping, DU Yuan-peng, GAO Zhen. Back-splicing Primers-based PCR Method for Specific Detection of circRNA [J]. Biotechnology Bulletin, 2022, 38(5): 279-285.
[2]	FAN Ya-peng, RUI Cun, ZHANG Yue-xin, CHEN Xiu-gui, LU Xu-ke, WANG Shuai, ZHANG Hong, XU Nan, WANG Jing, CHEN Chao, YE Wu-wei. Cloning,Expression and Preliminary Bioinformatics Analysis of the Alkaline Tolerant Gene GhZAT12 in Gossypium hirsutum [J]. Biotechnology Bulletin, 2021, 37(8): 121-130.
[3]	ZHANG Jing, XIONG Yan, HUA Yong-lin, GUO Yu, XIONG Xian-rong, ZI Xiang-dong, LI Jian. Screening of Reference Genes for Quantitative PCR of Skeletal Muscle Fiber Types in Mice [J]. Biotechnology Bulletin, 2021, 37(2): 71-79.
[4]	PANG Peng-xiang, CHANG Yan-nan, YU Rui-min, GAO Gang. Cloning,Expression and Bioinformatics Analyses of StSRP1 Gene in Solanum tuberosum [J]. Biotechnology Bulletin, 2019, 35(7): 10-16.
[5]	ZHU Rui, YE Yu-qing, WANG Ya-xin, YANG Chen-ru, WANG Hong-wei, SUN Xiao-qing, ZHANG Yan, LI Shang-qi, LI Jiong-tang. Cloning,Expression and Comparative Analysis of Two Progesterone Receptor Genes in Cyprinus carpio [J]. Biotechnology Bulletin, 2019, 35(7): 46-53.
[6]	WANG Xin-yan, ZHANG Jun-ling, SHI Zhi-yi. Molecular Characterization and Tissue Expression of cbx2 Gene in Paralichthys olivaceus [J]. Biotechnology Bulletin, 2019, 35(4): 69-75.
[7]	MAO Pei-qi, LI Hou-hua, LI Ai, CAO Zhi-xiu, HAN Mei-ling, ZHANG Yan-long. Effects of Silver Nitrate on the Synthesis of Phenolic Compounds and the Expression Levels of Related Genes in Callus Browning Process of Paeonia ostii ‘Fengdan’ [J]. Biotechnology Bulletin, 2018, 34(8): 101-107.
[8]	GENG Long-po, WANG Xin-wang, HUANG Lu-hua, DENG Ji-li, WANG Shi-hua, ZHANG Feng. Expression Analysis of Ribosomal Protein Genes in Aspergillus flavus [J]. Biotechnology Bulletin, 2018, 34(4): 194-200.
[9]	Hou Lan-fei, Yang Hong, Deng Zhi, Dai Long-jun, Men Zhong-hua, Li De-jun. Cloning,Expression and Bioinformatics Analyses of an ADC1 Gene in Hevea brasiliensis [J]. Biotechnology Bulletin, 2018, 34(11): 111-119.
[10]	LU Jia, DENG Qiu-ping, REN Wen-hua. Mechanism of Antimicrobial Peptide Scolopin 2-NH2 Isolated from Scolopendra subspinipes mutilans [J]. Biotechnology Bulletin, 2018, 34(11): 179-190.
[11]	KONG Wei-huan, Wang Jing-jing, WAN Peng-cheng, SHI Guo-qing. Establishment of a Duplex RT-PCR Detection Method for Porcine PEDV and PDC_OV [J]. Biotechnology Bulletin, 2018, 34(11): 205-209.
[12]	YU Li-li, LIN Peng, GUO Song-lin, WANG Yi-lei, ZHANG Zi-ping, WANG Ting-ting, FENG Jian-jun. Molecular Cloning and Immune Function Analysis of TLR3 Gene in Anguilla japonica [J]. Biotechnology Bulletin, 2018, 34(1): 160-171.
[13]	HUANG Kun-yong,LI Shan-shan,GUO Qiang,MAO Pei-chun,TIAN Xiao-xia,MENG Lin. Cloning and Expression Analysis of MoSOS1 Gene in Melilotus officinalis [J]. Biotechnology Bulletin, 2017, 33(9): 120-130.
[14]	TANG Hui-rong, CUI Jin ,ZHANG Jia-hua, DAI You-guo ,LI Wei-ming ,LIAO Chen. Effects of PDSW Combined with Hyperthermia on COX-2 and Bcl-2 Expressions in Gastric Cancer Cells and Tissues [J]. Biotechnology Bulletin, 2017, 33(7): 224-230.
[15]	GUO Tian-lu, ZHANG Huan-huan, DU Jian-zhong, HAO Yao-shan, WANG Yi-xue, SUN Yi. mRNA Expression and Bioinformatics Analysis of Cysteine Synthase in Allium sativum [J]. Biotechnology Bulletin, 2016, 32(8): 96-102.