Expression Analysis and Subcellular Localization of the MaBTB Gene in Banana

Abstract

Abstract: Real-time quantitative PCR of postharvest banana revealed that MaBTB exhibited differential expression patterns that are associated with ethylene biosynthesis. In naturally ripened bananas, expression of MaBTB was in accordance with ethylene biosynthesis. In contrast, for 1-methylcyclopropene-treated bananas, MaBTB expression levels remained constant. Following treatment with ethylene, MaBTB expression in banana fruit significantly increased with ethylene biosynthesis and peaked 3 d after harvest, which was 11 d earlier than that for naturally ripened banana fruits. These results suggested that MaBTB expression was induced by ethylene for regulation of postharvest banana ripening. Finally, subcellular localization assays showed that the MaBTB protein localizes to the nucleus.

Key words: Banana(Musa acuminataL. AAA group), MaBTB gene, Expression analysis, Subcelluar localization

Chai Wenlong, Jin Zhiqiang, Jia Caihong, Liu Juhua, Miao Hongxia, Zhang Jianbin, Xu Biyu,. Expression Analysis and Subcellular Localization of the MaBTB Gene in Banana[J]. Biotechnology Bulletin, 2014, 0(4): 71-76.

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