The Vector Construction of Anthocyanins as a Visual Marker Gene and Its Transient Expression in Maize Immature Embryos

Abstract

Abstract: Anthocyanin regulatory factor C1/Bperu genes are constructed respectively with a maize embryo specific promoter GLB1 and a constitutive promoter CaMV35S into plant transformation vectors pGlb1CB and p35SCB, and the recombinant expression vectors were introduced into maize embryos by particle bombardment. Microscopic observation confirmed that the two vectors were actively expressed in maize immature embryo cells. The use of anthocyanins as a marker gene not only could reduce the safety concerns from the public over GMO substantially, but could also be used as a visual indicator for the selection of transgenic plants, which could greatly simplify the screening procedures, improve the efficiency and reduce the costs.

Key words: Anthocyanins, Visual marker, Vector construction, Transient expression, Maize immature embryo

Zhao Xinmei, Wu Shubiao, Wang Yixue, Cui Guimei, Wang Xiaoqing, Du Jianzhong, Wang Xiaoli, Shang Yongjin, Sun Yi,. The Vector Construction of Anthocyanins as a Visual Marker Gene and Its Transient Expression in Maize Immature Embryos[J]. Biotechnology Bulletin, 2014, 0(4): 77-82.

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