Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (5): 179-183.

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Transposon Mutagenesis of Pseudomonas sp. BS1 and Preliminary Screening of High-producing Biosurfactant Mutant

Hou Jumei1 Han Wei1 Guo Liling2 Liu Tong2 Wang Yanjie3   

  1. (1. Supervision &Testing Center for Agricultural Product Quality,Ministry of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319;2. Institute of Plant Pathology and Applied Microbiology,Agriculture School,Heilongjiang Bayi Agricultural University,Daqing 163319;3. College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)
  • Received:2014-02-26 Online:2014-05-23 Published:2014-05-24

Abstract: The protoplast of Pseudomonas sp. BS1 was transformed by temperature-sensitive plasmid pTV1-OK that harbor Tn917. 3 stable transformants were obtained from the LB plates with Kan and Erm. Lots of mutants were obtained by transposon mutagenesis of Tn917, sequencely a mutant library was constructed. A predicted fragment was amplified by PCR from 5 randomly selected mutants, which showed that Tn917 successful inserted into the genome of Pseudomonas sp. BS1. E24 of a mutant from 24 selected randomly reach up 67% by the measurement of the emulsification index, significantly higher than wild-type strain. These results indicated that the transposon mutagenesis is an effective method for obtaining high-producing biosurfactant.

Key words: Protoplast, Transposon, Emulsification, Biosurfactant