Advances on Efficient and Precise Targeted Foreign DNA Integration

doi:10.13560/j.cnki.biotech.bull.1985.2019-1207

Abstract

Abstract: Precise and efficient target gene integration is a transgenic technology that inserts foreign DNA into the aimed position of cell genome. Integration of the target gene is achieved by the recombination of the homologous sequences of both cell genomic DNA and foreign DNA in the early stage. With the development of gene editing technology,especially the emergence of CRISPR/Cas9,the efficient and precise technology of foreign DNA integration becomes more and more mature and could be widely used in the researches on functional genomics,transgenic animals and genetic disease treatment. Here,we reviewed the research advances on precise target and efficient integration technologies such as gene editing,guided RNA modification technology,single base integration technology,transposon technology,and foreign DNA typing efficiency.

Key words: foreign DNA integration, precise and efficient, CRISPR/Cas9, single base integration, transposon technology

WU Zhi-sheng, FU Gao-hui, LUO Wen-jun, LIU Jun-jie, CHEN Hui-fang, BAI Yin-shan. Advances on Efficient and Precise Targeted Foreign DNA Integration[J]. Biotechnology Bulletin, 2020, 36(3): 29-37.

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