Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (7): 150-155.

• Research Report • Previous Articles     Next Articles

Isolation, Identification and Quorum Sensing of a Serratia spp.

Zhang Caili, Zhu Suqin, Wang Ying, Sun Xiujiao, Zeng Mingyong   

  1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003
  • Received:2013-12-18 Online:2014-07-15 Published:2014-07-16

Abstract: One quorum sensing strain was isolated from spoilage Litopenaeus vannamei. Specie was determined by 16S rRNA gene analysis and classical tests, it can not produce red pigment on medium, named it Serratia marcescens AK1.Quorum sensing was detected by reporter strains and signal molecules types were identified by thin layer chromatography. Quantitative analysis of the signaling molecule secreted by AK1 during different growth stages. The result indicates strain AK1 can induce Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136 produce purple and blue color respectively. Thin layer chromatography showed that AK1 can produce C6-HSL and 3-oxo-C6-HSL types of signaling molecules with density-dependent. They reached the maximum in the late logarithmic phase.

Key words: Serratia marcescens, Identification, Quorum sensing, N-acyl-homoserine lactones