Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (7): 156-161.

• Research Report • Previous Articles     Next Articles

Isolation of a Biosurfactant Producing Strain and Property Study

Wang Rui, Wu Juan   

  1. School of Resources and Environmental Engineering, Anhui University, Hefei 230601
  • Received:2013-12-09 Online:2014-07-15 Published:2014-07-16

Abstract: A biosurfactant-producing strain H1 was screened with blue agar plat from polluted soil of an oil field in China. The physiological and biochemical tests and phylogenetic analysis of 16S rDNA sequence showed the similarity of 99% between strain H1 and Klebsiella pneumoniae. The experiments showed that strain H1 could grow well and produce more biosurfactant under the following conditions:sucrose as carbon source, ammonium nitrate as nitrogen source, initial pH7.0 and 30℃.

Key words: Biosurfactant, Isolation, Surface tension, Production