Isolation, Identification and Enzyme Properties of a Strain Producing Feruloyl Esterases

Abstract

Abstract: Using the method of transparent zone, 37 filamentous fungi producing feruloyl esterase（FAE）were selected from rot soil samples containing wood fibers. The strain HA4087 was isolated by its high FAE activities. It was identified as Alternaria alternata by conidia morphology, 18S rDNA sequence and ITS sequence analysis. The characteristics of crude feruloyl esterase from A. alternate HA4087 were preliminarily studied. FAE activity of the crude enzyme was 86 mU/mL. The optimal temperature and pH for crude FAE was 55℃ and pH5.5, respectively. Crude FAE from A. alternate HA4087 remained nearly 90% of its maximal activity at 70℃ and was active in the pH range of 3.0-7.0. The strain producing thermostable and high active FAE provided a prerequisite for the industrialization of feruloyl esterase.

Key words: Feruloyl esterase, Identification, Alternaria alternata, Enzyme properties

Hu Hongyu, Zhou Yanyan, Chen Jing, Hou Yunhua. Isolation, Identification and Enzyme Properties of a Strain Producing Feruloyl Esterases[J]. Biotechnology Bulletin, 2014, 0(7): 162-167.

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