Optimization of Fermentation Conditions of Antagonistic Bacterium AM53 Against Colletotrichum gloeosporioides

Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (8): 164-168.

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Optimization of Fermentation Conditions of Antagonistic Bacterium AM53 Against Colletotrichum gloeosporioides

Yang Jing, Zhou Guoying, Tan Yimin, Lu Zongyan

The Key Laboratory for Economic Forest Cultivation and Conservation of Education Ministry, Central South University of Forestry and Technology, Changsha 410004

Revised:2013-12-10 Online:2014-08-15 Published:2014-08-01
Contact: 通讯作者: 周国英，女，博士，教授，研究方向：林业微生物；E-mail：gyzhou2118@163.com
Supported by:
林业公益性行业科研专项(201004014)

Abstract

Abstract: It was to optimize the fermentation conditions of Bacillus licheniformis. The Plackett-Burman design was used to optimize the fermentation conditions, and the results showed that culture temperature, the initial pH and the rotating speed had significant influence on the count of the living AM53. After the steepest ascent test and Box-Behnken design, the optimal fermentation conditions of AM53 that we got were：culture temperature was 30.5℃, the initial pH was 8, rotating speed was 185 r/min and inoculation volume was 2%, cultured 24 hours. Under this condition, the average of OD₆₀₀ was 1.861, which means the experimental values agreed with the predicted values, the predicted model was reliable and available for the optimization of AM53 fermentation conditions

Key words: Antagonistic bacterium, AM53, Fermentation conditions, Plackett-Burman design, Box-Behnken design

Yang Jing, Zhou Guoying, Tan Yimin, Lu Zongyan. Optimization of Fermentation Conditions of Antagonistic Bacterium AM53 Against Colletotrichum gloeosporioides[J]. Biotechnology Bulletin, 2014, 0(8): 164-168.

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Optimization of Fermentation Conditions of Antagonistic Bacterium AM53 Against Colletotrichum gloeosporioides

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