A PCR-RFLP Method for Channa argus and Channa maculate Discrimination

Abstract

Abstract:

In order to identificate Channa argus and C. maculate efficiently. We developed a PCR-RFLP method to conduct research in molecular biology. Briefly, two genomes of C. argus and C. maculate were aligned and compared, a SNP was identified which can discriminate two Channa species. A pair of primers was designed to amplify the mitochondrial DNA of both species, then digested by restriction enzyme EcoR I and test the digestion result by agarose gel of 1.5%. The result showed that the PCR product of C. maculate was digested by EcoR I that generate two fragments of 315 bp and 875 bp, while the PCR product of C. argus can not be digested by EcoR I. Thus, two Channa species were clearly discriminated.

Key words: Channa argus, Channa maculate, mtDNA, PCR-RFLP, Identification

Dong Chuanju, Zhang Songhao, Chen Kunci, Song Yingnan, Xu Peng, Sun Xiaowen. A PCR-RFLP Method for Channa argus and Channa maculate Discrimination[J]. Biotechnology Bulletin, 2014, 0(8): 59-64.

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