The Research on Mutagenesis Breeding of Phaeodactylum tricornutum and the Condition of EPA Producing

Abstract

Abstract: In order to obtain algal strains of high EPA production, Phaeodactylum tricornutum was mutated by 0.6% EMS. The mutant strain EP1 was isolated by single cell clone from 200 mutant clones. The EPA yield of EP1 was increased by 19.81% compared with the parent strain. The most suitable foster condition includes NaNO3 75 mg/L, pH7.5, day-night temperature 17-15℃, 12% inoculation quantity and training 7 days. The EPA yield of EP1 could reach 26.77 mg/g under the suitable foster condition. The results of subculture test showed that the mutant strain possesses good hereditary stability.

Key words: Phaeodactylum tricornutum , EMS mutagenesis , EPA

Liu Hongquan, Lin Xiaoyuan, Li Jieqiong, Pan Yihua. The Research on Mutagenesis Breeding of Phaeodactylum tricornutum and the Condition of EPA Producing[J]. Biotechnology Bulletin, 2014, 0(9): 114-119.

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