Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (9): 109-113.

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Screening of a Fungus with Insecticidal Activity and Measure of the Stability of Its Bioactive Compound

He Haibo1,3,Zhong Juan1,2,3,Yang Jie1,2,Zhou Jinyan1,2,Tan Hong1,2   

  1. 1. Key Laboratory of Environmental and Applied Microbiology，Chengdu Institute of Biology，Chinese Academy of Sciences，Chengdu 610041;
    2. Environmental Microbiology Key Laboratory of Sichuan Province，Chengdu 610041;
    3. University of Chinese Academy of Science，Beijing 100049
  • Received:2014-02-12 Online:2014-09-15 Published:2014-09-07

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Abstract: Using Brine Shrimp as primary screening assay and Aphid as second screening assay, the YLZ42 strain with insecticidal activity were isolated and screened from the soil in Yulin, Guangxi Province. According to the culture features, morphological characteristics, 18S rDNA and ITS sequence analysis, YLZ42 was identified as Bionectria ochroleuca. The ethyl acetate extract of YLZ42 fermentation broth have insecticidal activity to Plutella xylostella, Helicoverpa armigera and Laphygma exiguat. Results of bioassay showed that the corrected mortalities rate were more than 78.9%. The fermentation broth have high insecticidal activity under 100℃ for 60 min or pH1-11.