Clone and Expression of MeASR Gene in Cassava

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.021

Abstract

Abstract: Abscisic acid-stress-ripening induced protein（ASR）plays an important role in response to abiotic stresses. In the present study we isolated a first ASR gene designated MeASR from cassava. Sequence analysis showed that the open reading frame of MeASR gene contained 330 bp, encoding 109 amino acids. Multiple sequence alignment and phylogenetic analysis indicated that MeASR protein contained the conserved domains as ASR family had, and had a close genetic relationship with SlASR4 of tomato. Subcellular location assay showed that MeASR protein was localized in nucleus. Real-time quantitative PCR assay revealed that expression of MeASR was induced by osmotic stress and ABA treatments. These results suggested that MeASR might function as a transcription factor to involve in response to drought stress and ABA-signal regulation in cassava.

Key words: cassava, MeASR gene, gene clone, expression analysis, subcellular location

Hu Wei, Yan Yan, Wei Yunxie, Wang Wenquan, Xia Zhiqiang, Lu Cheng, Hou Xiaowan, Peng Ming. Clone and Expression of MeASR Gene in Cassava[J]. Biotechnology Bulletin, 2015, 31(10): 125-130.

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