Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (12): 243-248.doi: 10.13560/j.cnki.biotech.bull.1985.2015.12.035

• Research report • Previous Articles     Next Articles

The Identification and Analysis of Pathogenic Serratia marcescens from Locusta migratoria manilensis

Wu Nan, Guo Yangliu, Wang Xiaoshan, Li Yanyun, Lu Huipeng, Chen Cuizhen, Zhang Donglin, Fang Hai   

  1. Hebei Normal University of Science and Technology,Qinhuangdao 066000
  • Received:2015-04-15 Online:2015-12-19 Published:2015-12-19

Abstract: The effective isolation and identification of pathogen bacteria Serratia marcescens has a certain reference and application value for the control of infectious disease in culturing locusts caused by the S. marcescens. In addition, it will provide an insight for the research on biological control of plague of locust. From the dead Locusta migratoria manilensis from the infection, 10 strains of bacteria were isolated, and they were examined and analyzed with the apparent indications of taxonomy, 16S rRNA gene sequences and phylogenetic analysis, and artificial model of infection. Based on genetic and phenotypic information, the isolates were identified as S. marcescens which had high pathogenicity to cultured L. mingratoria manilensis. In conclusion, S. marcescens causes pathogenicity to L. mingratoria manilensis and it is a pathogenic bacterium in the culture of L. mingratoria manilensis, which lays a theoretical foundation for the biocontrol of locusts.

Key words: Locusta migratoria manilensis, Serratia marcescens, identification and analysis