Expression, Purification and Immunoprotection Potential of  Pseudomonas aeruginosa Outer Membrane Lipoprotein I

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.032

Abstract

Abstract: Outer membrane lipoprotein I(OprI)in Pseudomonas aeruginosa has solid immunogenicity and holds prospects in the development of vaccine. The OprI-expressing strain was obtained by molecular cloning. Western blotting verified that antibody specifically combined with OprI. The purified OprI was gained by the SDS-PAGE gel slices. Immunizing mice with OprI, specific immunity activated by OprI protected 57. 14% mice from P. aeruginosa infection, which reached significant protection compared with the control group. The orthogonal experiment was employed to obtain the optimal inducing expressing condition of OprI strain：strain OD₆₀₀ value，IPTG final concentration，inducing time and temperature，were 0. 8，0. 3 mmol/L，8 h，and 28℃，respectively；the optimal culture condition was that the rotation rate，glucose concentration，and medium volume were 230 r/min，0% and 50 mL，respectively. This work laid the groundwork for the researches on industrial fermentation，protein function and vaccine development of OprI.

Key words: Pseudomonas aeruginosa, OprI protein, immunoprotection, orthogonal experiment, induction conditions

Chen Chunlin, Liu Xiang, Wang Chengxiang, Zhang Xiaojuan, Ju Xiong, Zhang Tao, Wu Sanqiao. Expression, Purification and Immunoprotection Potential of Pseudomonas aeruginosa Outer Membrane Lipoprotein I[J]. Biotechnology Bulletin, 2015, 31(7): 207-213.

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Expression, Purification and Immunoprotection Potential of Pseudomonas aeruginosa Outer Membrane Lipoprotein I

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