Isolation and Identification of Novel Denitrifier Strain Rhizobium radiobacter and Its Nitrifying Characterisation

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.026

Abstract

Abstract: In order to screen and identify new strain with nitrification, a denitrifier was isolated from sewage treatment plant. The strain named N312 isolated by enrichment and plate culture was identified based on its morphology characteristics, 16S rDNA gene sequence analysis and Biolog. The conditions of medium composition were optimized by orthogonal experiment. The autotrophic denitrifier strain N312 was isolated and identified as Rhizobium radiobacter, which was rod, Gram-negative, colony round and white. The results indicated that the nitrite removal efficiency by the strain could reach 100% after 6 days. The best medium was NaNO₂ 1.5 g, Na₂CO₃ 1.5 g, Mg₂SO₄·7H₂O 0.25 g, FeSO₄·7H₂O 0.2 g，KH₂PO₄ 0.5 g. Strain N312 is a new autotrophic denitrifier with a highly research value.

Key words: denitrifier, 16S rDNA, Biolog, orthogonal experiment, nitrification rate

Luo Xiaoxi, Gao Jianzhong, Chen Zaizhong, Yu Yongliang. Isolation and Identification of Novel Denitrifier Strain Rhizobium radiobacter and Its Nitrifying Characterisation[J]. Biotechnology Bulletin, 2015, 31(5): 167-172.

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