The Construction of Expression Vector of Gene CBF from Vitis amurensis and the Effects of It on the Root Growth of Arabidopsis

doi:10.13560/j.cnki.biotech.bull.1985.2016.01.019

Abstract

Abstract: In order to investigate the response mechanism of CBF gene in the regulation of plant to the salt stress, the plant over-expression vectors of VaCBF1, VaCBF2 and VaCBF3 from Vitis amurensis were constructed. The results by restriction enzyme digestion and agarose gel electrophoresis showed that 3 genes were correctly inserted into pBASTA, indicating that the expression vectors were successfully constructed. Then, 3 over-expressed vectors were transformed into Agrobacterimu tumefaciens EHA105, and Arabidopsis thaliana was leached using floral dip method. The transgenic plants of A. thaliana with over-expression of 3 genes were screened by herbicide Basta. Furthermore, wild and transgenic plants were treated with salt stress. The results revealed that the elongation length of primary root of OE-CBF2 transgenic plant was longer than others, and the lateral roots in 3 genes transgenic plants were longer than wild ones. These results indicate that the CBF genes of V. amurensis play the critical regulation role in the root development during salt stress.

Key words: Vitis amurensis, CBF gene, expression vector, root growth

WANG Fa-wei, DONG Jin-ye, LI Xiao-wei, LIU Yang, WU Xue-yan, WANG Nan, DONG Yuan-yuan, CHEN Huan, LI Hai-yan. The Construction of Expression Vector of Gene CBF from Vitis amurensis and the Effects of It on the Root Growth of Arabidopsis[J]. Biotechnology Bulletin, 2016, 32(1): 109-114.

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