Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (6): 120-128.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1188
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GAO Cong(), XIAO Chu-jian, LU Shuai, WANG Su-rong, YUAN Hui-hua(), CAO Yun-ying()
Received:
2021-09-14
Online:
2022-06-26
Published:
2022-07-11
Contact:
YUAN Hui-hua,CAO Yun-ying
E-mail:GC1909310005@sohu.com;cyy@ntu.edu.cn;yuanhh@ntu.edu.cn
GAO Cong, XIAO Chu-jian, LU Shuai, WANG Su-rong, YUAN Hui-hua, CAO Yun-ying. Promoting Effect of Graphene Oxide on the Root Growth of Arabidopsis thaliana[J]. Biotechnology Bulletin, 2022, 38(6): 120-128.
基因 Gene | 登录号 Locus | 引物Primer(5'-3') | 片段长度 Size/bp |
---|---|---|---|
ERFII-1 | At4g17490 | F:TTGTAGCAGCAGAGGAGAAGAG | 107 |
R:CCAAACACGAGTTCCACGAC | |||
ARF7 | At5g20730 | F: GCGGCTAAAACAAGAACTCG | 107 |
R:CGCCTCCATCTAAACCGTAA | |||
ARF19 | At1g19220 | F:TCCAGTGCTGCAATCAGTTC | 112 |
R:CCTCCACCATTCATGATTCC | |||
CKX1 | At2g41510 | F:ACAGAGGAAACAAGCCTACGAC | 102 |
R:TGACTTTGCGAGTTGGATGG | |||
ADC1 | At2g16500 | F:TGTGGCTTCGGTTAGGTTTG | 138 |
R:GTCTCATGTTGTTGACCAGCTG | |||
DAR2 | At2g39830 | F:AGCATGAGTTCTCTCTGTCAGG | 142 |
R:CCAAAACGGATGGCATCGATAC | |||
IQM3 | At3g52870 | F:GGAGGGTGATTGTTGACAATGG | 120 |
R:AGGTTCACTGCGTTCTCTCTG | |||
ACT2 | At3g18780 | F:GCCATCCAAGCTGTTCTCTC | 270 |
R:GCTCGTAGTCAACAGCAACAA |
Table 1 Primer sequences for fluorescence quantitative expression analysis
基因 Gene | 登录号 Locus | 引物Primer(5'-3') | 片段长度 Size/bp |
---|---|---|---|
ERFII-1 | At4g17490 | F:TTGTAGCAGCAGAGGAGAAGAG | 107 |
R:CCAAACACGAGTTCCACGAC | |||
ARF7 | At5g20730 | F: GCGGCTAAAACAAGAACTCG | 107 |
R:CGCCTCCATCTAAACCGTAA | |||
ARF19 | At1g19220 | F:TCCAGTGCTGCAATCAGTTC | 112 |
R:CCTCCACCATTCATGATTCC | |||
CKX1 | At2g41510 | F:ACAGAGGAAACAAGCCTACGAC | 102 |
R:TGACTTTGCGAGTTGGATGG | |||
ADC1 | At2g16500 | F:TGTGGCTTCGGTTAGGTTTG | 138 |
R:GTCTCATGTTGTTGACCAGCTG | |||
DAR2 | At2g39830 | F:AGCATGAGTTCTCTCTGTCAGG | 142 |
R:CCAAAACGGATGGCATCGATAC | |||
IQM3 | At3g52870 | F:GGAGGGTGATTGTTGACAATGG | 120 |
R:AGGTTCACTGCGTTCTCTCTG | |||
ACT2 | At3g18780 | F:GCCATCCAAGCTGTTCTCTC | 270 |
R:GCTCGTAGTCAACAGCAACAA |
Fig.2 Effects of different concentrations of GO on the tap-root growth of A. thaliana A:Representative photograph of A. thaliana taproot growth after 6 d of planting. B:Effects of GO on the taproot length. n=30. CK,20,50,100 and 200 refer to 0,20,50,100 and 200 μg/mL GO treatment,respectively. The error line on the column is the standard deviation,and the different small letters above the bars mean significant difference among treatments(P≤0.05). The experiment was repeated three times. The same below
Fig.3 Effects of different concentrations of GO on the lat-eral root growth of A. thaliana A:Representative photograph of A. thaliana lateral root growth after 11 d of planting. B:Effects of GO on the lateral root. n=15. CK,20,50,100 and 200 refer to 0,20,50,100 and 200 μg/mL GO treatment,respectively
Fig.4 Effects of different concentrations of GO on the different parts of taproot of A. thaliana A:Meristem zone length of taproot of A. thaliana growth after 4 d of planting. B:Extension zone length of taproot of A. thaliana growth after 4 d of planting. C:Diameter of taproot tip of A. thaliana growth after 4 d of planting. D:Length of taproot cap of A. thaliana growth after 4 d of planting. E:Representative photograph of A thaliana growth after 4 d of planting by confocal microscope staining with PID,n=30. 0 or CK and 50 refer to 0 and 50 μg/mL GO treatment,respectively. Asterisk above the bars indicate significant difference between the two treatments(P≤0.05). The same below. Scale bar is 5 μm
Fig.5 Tissue staining and SOD activity quantitative analysis of A. thaliana treated with different concentrations of GO A and C:0 μg/mL GO treatment as control. B and D:Treated with GO in 50 μg/mL concentration. A and B:Representative photograph of A. thaliana taproot tip with staining with TTC,scale bar is 5 mm. C and D:Representative photograph of A. thaliana seedlings with staining with NBT,scale bar is 2 mm. E:Quantitative analysis of SOD activity
Fig.6 Effects of different concentrations of GO on the gene expressions related to root growth in A. thaliana A:Regulation of root length related gene expression. B:Regulation of lateral root related gene expression. CK and 50 refer to 0 and 50 μg/mL GO treatment
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