The Differential Expression of Genes for Key Enzymes in Gibberellin Metabolism in Potato Mutant

doi:10.13560/j.cnki.biotech.bull.1985.2016.01.021

Abstract

Abstract: In order to reveal the correlation between the mutation of plant height and genes of key enzymes in gibberellin(GA)metabolic pathway, using the mutant strain 4P2-9 of potato as materials, we established double standard curve by relative quantitative RT-PCR, and tested the expression of GA metabolism-related genes in the “Plateau 4” and its mutant “4P2-9” using Actin gene as reference gene. The results showed that the plant height and the internode number of 4P2-9 significantly were lower than the control material(P<0.05);however, the internode length was significantly higher than the control(P<0.05), moreover the expressions of gene KS, KO and GID1 for key enzymes in GA metabolism were significantly down-regulated, and were 0.725, 0.657 and 0.890 times the control, respectively. The expressions of GA20ox1 and GA2ox1 genes were increased, and were 1.425 and 1.557 times the control, respectively. The results revealed that the expression changes of genes for key enzymes in the GA metabolism was one reason of the variation of 4P2-9 plant height, and the up-regulation of gene GA20ox1 and GA2ox1 expression was the main factor for the decrease of plant height.

Key words: potato, mutation of plant-height, GA, gene expression

SHI Jian-bin, YANG Yong-zhi, WANG Jian. The Differential Expression of Genes for Key Enzymes in Gibberellin Metabolism in Potato Mutant[J]. Biotechnology Bulletin, 2016, 32(1): 124-130.

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