Adjuvanticity of Cordyceps militaris stroma Polysaccharides in Inactivated Vaccine to Avian Infectious Bronchitis

Abstract

Abstract: It aimed to use Cordyceps militaris stroma polysaccharides as adjuvants that mix with inactivated vaccine to infectious bronchitis，and to investigate its effects on the immune functions of broilers. Total 150 1-day Huangyu broilers were selected and randomly divided into 5 groups. Then the broilers of 21 d after immunized（35-day）were infected with Mass 41 infectious bronchitis virus（IBV M41）strain by the ocular-nasal route. The proliferation of peripheral blood mononuclear cells（PBMC），serum antibody titer，the protection of IBV virulent strain，and histopathological changes in each group were evaluated respectively by MTT method and hemagglutination inhibition assay. The results showed that proliferation index of PBMC and serum antibody titer in the broilers with dosage C. militaris stroma polysaccharides as adjuvants significantly raised（P<0.05）. Following challenge test with IBV M41 strain，broilers inoculated with C. militaris stroma polysaccharides showed significantly lighter clinical symptoms，there were no histopathological changes in lung and kidney，and immune protection reached 96.7%. This indicated that C. militaris stroma polysaccharides as adjuvants increased the immunity of broilers to infectious bronchitis.

Key words: Cordyceps militaris stroma polysaccharides, avian infectious bronchitis, adjuvants

CLC Number:

10.13560/j.cnki.biotech.bull.1985.2016.12.018

ZHOU Mei-xian,ZHOU Ye-fei. Adjuvanticity of Cordyceps militaris stroma Polysaccharides in Inactivated Vaccine to Avian Infectious Bronchitis[J]. Biotechnology Bulletin, 2016, 32(12): 108-112.

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