Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (12): 113-123.

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Molecular Cloning and Expression Analysis of Two Types of perlucin Gene from Small Abalone Haliotis diversicolor

LIN Shi,ZHANG Li-li,WANG Guo-dong   

  1. Fisheries College,Jimei University,Key Laboratory of Healthy Mariculture for the East China Sea,Ministry of Agriculture,Xiamen 361021
  • Received:2016-04-26 Online:2016-12-25 Published:2016-12-07

Abstract: SMART RACE technology was employed to clone the full-length cDNA sequences of 2 Manna-binding lectin(MBL)members of perlucin1 and perlucin4 in Haliotis diversicolor. Real-time PCR were used to analyze their expression patterns in the different tissues of H. diversicolor. As results,the full length cDNA of perlucin1 was 668 bp,and the deduced protein was composed of 165 amino acids,with two disulfide bonds,two glycosylation sites and 13 phosphorylation sites;its predicted molecular weight was about 18.8 kD,and pI was about 4.57. The full length cDNA of perlucin4 was 587 bp and the deduced protein was composed of 156 amino acids,with two disulfide bonds,one glycosylation sites and 7 phosphorylation sites;its predicted molecular weight was about 17.8 kD,and pI was about 4.43. Gene perlucin1 and perlucin4 exclusively expressed only in hepatopancreas among 7 tissues of gill,hepatopancreas,blood lymph,up foot,mantle,mucous gland,and kidney. The above results indicate that perlucin genes involved in innate immune defense mechanisms and play a key role in immune recognition of H. diversicolor.

Key words: perlucin lectin, mannan-binding lectin, Real-time PCR

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