Construction of Controlled Inducible Expression System in Streptococcus zooepidemicus ATCC39920 and Its Application

Abstract

Abstract: This work aimed at establishing an inducible gene expression system in Streptococcus zooepidemicus，and investigating the application of it in controllable two-stage fermentation. Induced expression vectors by sucrose，lactose，xylose and nisin were constructed，then using hasA of hyaluronic acid（HA）synthase as reporter gene，and ?hasA with capsule-deletion resulted from the function deletion of gene hasA as the host bacterium，the feasibility of these systems was verified by observing the generation of capsule. The results showed that：The expression of hasA was not induced in the xylose and nisin vector，at low-efficiency in lactose vector，and efficient and rigorous in sucrose vector. A controllable two-stage fermentation mode was conducted using ΔhasA/pLH201 as strain and glucose only as sole sugar source for the growth of bacteria，and added sucrose induced the generation of HA，and HA production reached at 3.4 g/L. In conclusion，this study successfully constructed a sucrose-induced gene expression system that can be used for two-stage fermentation of S. zooepidemicus.

Key words: Streptococcus zooepidemicus, controlled inducible expression system, controllable two-stage fermentation, hyaluronic acid

CLC Number:

10.13560/j.cnki.biotech.bull.1985.2016.12.021

ZHENG Xiao-e,WANG Zhen,LIU Hao. Construction of Controlled Inducible Expression System in Streptococcus zooepidemicus ATCC39920 and Its Application[J]. Biotechnology Bulletin, 2016, 32(12): 130-136.

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