The Optimizationof FermentationCondition for Cellulase Production by Penicillium oxalicum

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.020

Abstract

Abstract: Cellulase plays an important role in the biological energy, textile, feed, paper industry, et al.Screening efficient strains and optimizing the fermentation condition are the effective way of obtaining cellulase.A cellulose-degrading strain JG was isolated from the soil containing rotten wood and straw.Morphology and molecular phylogenetic studies were used for its identification.Some factors during its fermentation were optimized, including initial pH, carbon source, nitrogen source and surfactants.The strain JG was identified as Penicillium oxalicum by analyzing its morphology and ITS(Internal Transcribesd Spacer)gene sequence phylogenetic systematics.Its optimal cultural conditions for the highest cellulase production were as following：initial pH values 2-3, carbon and nitrogen source were CMC-Na and soybean meal.Studying the effects of adding different kinds of surfactant on the fermentation process demonstrated that the lecithin of wide source and relatively low price had an obviously high effects on the cellulase production in JG, and the enzyme activities of FPA, CMCase, and BGL increased by 72.9%, 20.8% and 33.6% respectively.In conclusion, JG produced cellulase under lower initial pH, and lecithin enhanced its cellulase activity significantly.

Key words: Penicillium oxalicum, cellulase, lecithin, optimization

LI Yang, GAO Xiao-rong, ZHANG Jian. The Optimizationof FermentationCondition for Cellulase Production by Penicillium oxalicum[J]. Biotechnology Bulletin, 2016, 32(2): 152-157.

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