Preparation of Porous Biological Carrier with DHP-galactose Complex and Its Application in Culture of Human Hepatocytes

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.037

Abstract

Abstract: In order to enhance the biocompatibility of dehydrogenation polymer(DHP)with human hepatocytes，the DHP was copolymerized with D-galactose，by which a porous biological carrier for medical use was prepared using the method of hydrogel. Fourier transform infrared spectrometer(FT-IR)，nuclear magnetic resonance(NMR)spectroscopy，BET specific surface area determination，and scanning electron microscope(SEM)were applied to elucidate the main composition，chemical and physical structure，and morphology of the porous biological carrier. Inverted microscope and albumin and glucose kits were used to check the morphology and metabolic activity of cultured hepatocytes. The results indicated that the porous biological carrier with excellent performance was prepared with co-polymer of DHP and D-galactose as raw material，and the specific surface area was 6.013 m²/g. The human hepatocytes healthily adhered on the porous biological carrier to grow and proliferate while the carriers were used to culture human hepatocytes. The maximum values of albumin secretion and glucose consumption using biological carrier containing galactose were 9.85 g/(d·L)and 16.134 mmol/(d·L)，respectively，demonstrating that the hepatocytes presented high metabolic activity during culturing，and the porous biological carrier prepared with DHP-galactose complex had a great biocompatibility.

Key words: DHP, biological carrier, human hepatocytes, culture, biocompatibility

WU Chen-xi, XIE Yi-min, YE Zhe-zi, WANG Peng, LE Xi. Preparation of Porous Biological Carrier with DHP-galactose Complex and Its Application in Culture of Human Hepatocytes[J]. Biotechnology Bulletin, 2016, 32(6): 250-257.

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