Cloning and Expression Pattern Analysis of NBS-LRR Like-gene in Peanut

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.014

Abstract

Abstract: Based on peanut’s transcriptome and genome data,a full length sequence of NBS-LRR like-gene was cloned. This gene,which showed 79% similarity with disease resistance protein（KHN40407.1）of Glycine soja,was designated as AhNDrp gene. The length of cDNA of AhNDrp gene was 2 832 bp,and contained no intron. AhNDrp gene harbored 5 typical conserved domains with resistance-related protein,and Domain V was repeat sequence with rich leucine. Real-time quantitative PCR analysis showed that AhNDrp gene was expressed exclusively in root,and the expression was significantly increased after aflatoxin treatment,indicating that this gene was involved in the resistance response.

Key words: peanut, NBS-LRR gene, gene clone, expression analysis

FENG Yan-fang, GENG Li-li, HAN Rong, ZHANG Jie. Cloning and Expression Pattern Analysis of NBS-LRR Like-gene in Peanut[J]. Biotechnology Bulletin, 2016, 32(8): 90-95.

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