Functional Analysis of Soybean GmAP1

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.013

Abstract

Abstract: The full length GmAP1 gene,a homologous gene of AP1,was cloned from the soybean cultivar Tianlong 1 based on the putative sequence of soybean genome in Glycine max Wm82.a2.v1database. GmAP1 was 711 bp in length and encoded a protein of 236 amino acids. The structure analysis of protein sequence showed that GmAP1 protein characterized as MADs-box gene family. To analyze the function of GmAP1,the expression patterns of GmAP1 in different floral organs were analyzed by RT-PCR,and GmAP1 was overexpressed in Arabidopsis. The results showed that GmAP1differently expressed in different floral organs with the highest level in sepals,following by the petals. Transgenic Arabidopsis plants displayed phenotypes of early flowering,dwarf,and increasing number of sepals,petals,and stamens. The result indicated that GmAP1 was one of AP1 homologous genes in soybean with conserved function and played an important role in flowering and floral organ development morphogenesis.

Key words: flowering, sepals, GmAP1, MADS gene, soybean

SONG La-la, ZHANG Xiao-mei, CHEN Fu-lu, FU Yong-Fu, ZHAO De-gang. Functional Analysis of Soybean GmAP1[J]. Biotechnology Bulletin, 2016, 32(8): 84-89.

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