Green Fluorescent Protein Marker of Biocontrol Streptomyces SSD49 and Its Colonization on the Populus tomentosa Somaclone

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.026

Abstract

Abstract: Poplar canker is one of the major biohazard of poplar plantation in China,and the sustained and effective method to control poplar canker is biological control. This study aims to obtain a strain which has solid bio-control effect on Botryosphaeria dothidea and can colonize in poplar efficiently. This study imported a strong promoter（ermEp）and constructed highly-expressed green fluorescent protein（GFP）recombinant plasmid,then led the plasmid into bio-control strain Streptomyces SSD49 through joint experiment,and constructed the GFP-tagged strain SSD49-pIJ8660Ep. Fluorescence microscope was used to study the colonization of this biocontrol strain in Populus tomentosa somaclone. Results showed that the GFP-tagged SSD49 didn’t affect its bacteriostatic activity against poplar canker pathogen and colonized in stems and leaves of P. tomentosa somaclone. This study successfully constructed the GFP-tagged strain SSD49,and the strain presented certain colonization in P. tomentosa somaclone.

Key words: green fluorescent protein marker, colonization, Streptomyces, Botryosphaeria dothidea

LIU Xiao-yu, MA Yu-chao. Green Fluorescent Protein Marker of Biocontrol Streptomyces SSD49 and Its Colonization on the Populus tomentosa Somaclone[J]. Biotechnology Bulletin, 2016, 32(9): 197-202.

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