Screening and Identification of a Marine Bacterium Producing Chitin Deacetylase and Optimization of Its Fermentation Condition

doi:10.13560/j.cnki.biotech.bull.1985.2017-0413

Abstract

Abstract: A strain MCDA02 producing chitin deacetylase was screened with the media added nitro-N-acetyl aniline from the marine mud of Haizhou bay. Then,it was identified as Microbacterium keratanolyticum through morphological,biochemical characteristics,and 16S rDNA sequence analysis. With single-factor strategy and orthogonal experiments,the fermentation condition was optimized as followings：fermentation period of 48 h,initial pH of 7.0,culture temperature of 30℃,50 mL of liquid medium in a 250 mL flask,and 3% chitin. Under the optimized conditions,the highest chitin deacetylase activity of strain MCDA02 reached 158.47 U/mL,which was about 3.2 times of that before optimization. The results laid a foundation for further study of the chitin deacetylase from the strain MCDA02.

Key words: chitin deacetylase, marine bacteria, identification, fermentation condition

LAI Jiang-li, LIU Shu, HU Sheng-yuan, GU Zhang-hui, WANG Shu-jun, FANG Yao-wei. Screening and Identification of a Marine Bacterium Producing Chitin Deacetylase and Optimization of Its Fermentation Condition[J]. Biotechnology Bulletin, 2017, 33(11): 153-159.

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