[1] Maeda H, Dudareva N. The shikimate pathway and aromatic amino Acid biosynthesis in plants[J]. Annu Rev Plant Biol, 2012, 63:73-105. [2] Steinrucken HC, Amrhein N. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase[J]. Biochem Biophys Res Commun, 1980, 94(4):1207-1212. [3] Ghanizadeh H, Harrington KC, James TK, et al. Mechanisms of glyphosate resistance in two perennial ryegrass(Lolium perenne)populations[J]. Pest Manag Sci, 2015, 71(12):1617-1622. [4] González-Torralva F, Brown AP, Chivasa S. Comparative proteomic analysis of horseweed(Conyza canadensis)biotypes identifies candidate proteins for glyphosate resistance[J]. Sci Rep, 2017 Feb 15;7:42565. doi:10. 1038/srep42565. [5] Molin WT, Wright AA, Lawton-Rauh A, et al. The unique genomic landscape surrounding the EPSPS gene in glyphosate resistant Amaranthus palmeri:a repetitive path to resistance[J]. BMC Genomics, 2017, 18(1):91. [6] Gaines TA, Barker AL, Patterson EL. EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia[J]. PLoS One, 2016, 11(12):e0168295. [7] Harrison LA, Bailey MR, Naylor MW, et al. The expressed protein in glyphosate-tolerant soybean 5-Enolypyruvylshikimate-3- Phosphate synthase from Agrobacterium sp. strain CP4, is rapidly digested in vitro and is not toxic to acutely gavaged mice?[J]. J Nutr, 1996, 126(3):728-740. [8] Marani MM, Costa J, Mafra I, et al. In silico peptide prediction for antibody generation to recognize 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS)in genetically modified organisms[J]. Biopolymers, 2015, 104(2):91-100. [9] Yin KF, Liu AR, Li SG, Li Mi, et al. Construction of iron-polymer-graphene nanocomposites with low nonspecific adsorption and strong quenching ability for competitive immunofluorescent detection of biomarkers in GM crops[J]. Biosens Bioelectron, 2017, 90:321-328. [10] 王文治, 杨本鹏, 蔡文伟, 等. 抗除草剂bar基因与EPSPS基因在转基因甘蔗中的应用研究[J]. 生物技术通报, 2016, 32(3):73-78. [11] Chhapekar S, Raghavendrarao S, Pavan G, et al. Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate[J]. Plant Cell Rep, 2015, 34(5):721-731. [12] Howe AR, Gasser CS, Brown SM, et al. Glyphosate as a selective agent for the production of fertile transgenic maize(Zea mays L.)plant[J]. Mol Breeding, 2002, 10(3):153-164. [13] Zhao FY, Li YF, Xu P, et al. Agrobacterium-mediated transformation of cotton(Gossypium hirsutum L. cv. Zhongmian 35)using glyphosate as a selectable marker[J]. Biotechnol Lett, 2006, 28(15):1199-1207. [14] Zhou H, Arrowsmith JW, Fromm ME, et al. Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation[J]. Plant Cell Reports, 1995, 15(3-4):159-163. [15] 刘建强, 孙仲序, 赵春芝. 转基因植物鉴定方法的研究概况[J]. 山东林业科技, 2002, 5:39-43. [16] Paterson HA, Brubaker LC, Wendel FJ. A rapid method for extraction of cotton(Gossypium spp.)genomic DNA suitable for RFLP or PCR analysis[J]. Plant Mol Biol Reptr, 1993, 11:122-127. [17] 盖树鹏, 孟祥栋. 转基因植物的筛选与检测[J]. 山东农业大学学报:自然科学版, 2000, 31(1):95-100. [18] Kamle S, Ojha A, Kumar A. Development of enzyme-linked immunosorbent assay for the detection of Bt protein in transgenic cotton[J]. Methods Mol Biol, 2013, 958:131-138. |