Antimicrobial Activities and Efficacy of Endophytic Streptomyces sp. SSD49 in Plant Disease Control and Plant-growth-promoting

doi:10.13560/j.cnki.biotech.bull.1985.2016-1121

Abstract

Abstract: The endophytic Streptomyces sp. SSD49,isolated from the roots of Strophanthus divaricatus,showed strong antifungal activity against Botryosphaeria dothidea. In order to analyze its antimicrobial activity against various pathogens and the characterization of plant-growth-promoting as well as the potential of biocontrol agents,a series of experiments were conducted,including plat confrontation,tissue culture and potting test in greenhouse. The results showed that Streptomyces sp. SSD49 inhibited the bacterial pathogens of Cryphonectria parasitica,Sclerotinia sclerotiorum,Phytophthora capsici,Dothiorella gregaria,Lonsdalea quercina subsp. populi,as well as plant pathogens and human opportunistic pathogens of Methicillin-resistant Staphylococcus aureus and Candida albicans. It secreted chitinase and produced indole acetic acid at the concentration of 31.56 mg/L. The shoot height,root length,shoot and root dry weight of Populus tomentosa tissue seedlings,soybean,tomato and pepper plants inoculated with strain SSD49 increased by 16.98-41.82%,19.00-55.85%,4.58-70.87% and 15.25-126.06%,respectively. The inhibition rate to Sclerotinia sclerotiorum reached 88.24%. The above results demonstrated that endophytic Streptomyces sp. SSD49 is potentially applicable in controlling plant disease in agriculture and forestry,while the mechanism of plant disease control and plant-growth-promoting needs to be further studied.

Key words: endophyte, endophytic Streptomyces, antimicrobial activities, plant disease control, plant-growth-promoting

GE You-you, LIU Xiao-yu, DOU Gui-ming, MA Yu-chao. Antimicrobial Activities and Efficacy of Endophytic Streptomyces sp. SSD49 in Plant Disease Control and Plant-growth-promoting[J]. Biotechnology Bulletin, 2017, 33(6): 121-127.

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