Acid Domestication and UV Induction Increase Microalgae Tolerance to Acid Stress

doi:10.13560/j.cnki.biotech.bull.1985.2017-0446

Abstract

Abstract: Utilization of microalgae for direct fixation of carbon from coal-based flue gas may reduce CO₂ emissions，and also produce bio-based products with multiple values. However，CO_2，NOx and SO₂ at high concentration in the flue gas always cause excessive acidification of culture medium，leading to severe inhibition of microalgae growth. The present study was conducted to establish a simple and effective method of microalgae domestication for enhancing the microalgae resistance against acid stress and increasing their initial growth rate when inoculated in low-pH medium. The initial growth rate of the acid-domesticated and the UV-treated Scenedesmus reached 0.253 g/（d·L），which was significantly higher than that（0.132 g/（d·L））by only acid domestication at pH4.5 and that（0.092 g/（d·L））of 5-min induction at low-dose of UV radiation in low-pH environment. The more remarkable effects were achieved by combining such acid domestication and UV induction. The domesticated seed algae grew normally in the acidic environment of pH3，but the non-domesticated microalgae growth stopped. The initial growth rate of the domesticated seed algae was 6.6 times higher than that of the non-domesticated microalgae in low-pH environment. The above results revealed that acid domestication and UV induction significantly improved Scenedesmus tolerance against low-pH environment.

Key words: Scenedesmus, flue gas, acid domestication, biological carbon reduction

SHI Fei-fei, CHEN Tong, CHENG Wei-lan, SONG Cheng-Fei, JI Chun-li, LI Run-zhi. Acid Domestication and UV Induction Increase Microalgae Tolerance to Acid Stress[J]. Biotechnology Bulletin, 2017, 33(8): 146-151.

References

[1] 岳丽宏, 陈为公, 等. 烟气环境条件下小球藻的生长及其CO₂固定[J] . 青岛理工大学学报, 2005, 26（06）：15-19.
[2] 张一昕, 赵兵涛, 熊锴彬, 等. 微藻固定燃烧烟气中CO₂的研究进展[J] . 生物工程学报, 2011, 27（02）：164-171.
[3] Jiang Y L, Zhang W, Wang J F, et al. Utilization of simulated flue gas for cultivation of Scenedesmus dimorphus[J] . Bioresource Technology, 128（2013）359-364.
[4] Jiang YL, Peng XW, Zhang W, Liu TZ. Enhancement of acid resistance of Scenedesmus dimorphus by acid adaptation[J] . J Appl pHycol, 2012, 24：1637-1641.
[5] Yue LH, Chen WG. Isolation and determination of cultural characteristics of a new highly CO₂ tolerant fresh water microalgae[J] . Energy Conversion and Management, 2005, 46（11-12）1868-1876.
[6] Li FF, Yang ZH, Zeng R, et al. Microalgae capture of CO₂ from actual flue gas discharged from a combustion chamber[J] . Industrial & Engineering Chemistry Research, 2011, 50（11）：6496-6502.
[7] 蒋银莉 . 利用模拟烟道气培养产油微藻研究[D] . 青岛：中国科学院青岛生物能源与过程研究所, 2012.
[8] 刘琪. 不同PH值对栅藻生长增殖的影响[A] . 中国海洋湖沼学会藻类学分会. 中国海洋湖沼学会藻类学分会第七届会员大会暨第十四次学术讨论会论文摘要集[C] . 中国海洋湖沼学会藻类学分会, 2007：1.
[9] 张虎, 张桂艳, 温小斌, 等. pH对小球藻Chlorella sp. XQ-200419光合作用、生长和产油的影响[J] . 水生生物学报, 2014, 38（06）：1084-1091.
[10] 吴运东, 吴沿友, 李潜, 等. pH与氟对莱茵衣藻和蛋白核小球藻胞外碳酸酐酶活性及光合效率的影响[J] . 农业环境科学学报, 2011, 30（10）：1972-1977.
[11] 高文涛, 施云海, 李伟, 等. 利用微藻减排CO₂的研究进展[A] . 上海市化学化工学会2011年度学术年会论文集[C] . 2011：3.
[12] 杨闯. 紫外线诱变选育耐高浓度CO₂的微藻及不同株系的rbcL基因序列差异性分析[D] . 青岛：青岛理工大学, 2010.
[13] 张硕. 耐受高浓度CO₂、NO能源微藻的筛选及生长条件的优化[D] . 济南：山东大学, 2015.
[14] 杨瑾, 王铭, 等. 氮胁迫对雨生红球藻色素积累与抗氧化系统的影响[J] . 植物生理学报, 2011, 47（02）：147-152.
[15] 董爱玲, 颉建明, 李杰, 等. 低温驯化对低温胁迫下茄子幼苗生理活性的影响[J] . 甘肃农业大学学报, 2017, 52（01）：74-79.
[16] 饶本强, 吴沛沛, 李敦海, 等. 低温驯化和外源糖对爪哇伪枝藻冷胁迫的影响[J] . 生态科学, 2011, 30（02）：128-134.