Sequencing and Analyzing Mitochondrial Genome of Cacopsylla coccinea

doi:10.13560/j.cnki.biotech.bull.1985.2018-0717

Abstract

Abstract: In order to protect the medicinal plant resources such as Cacopsylla and to discover its biological properties，physiological and biochemical characteristics，we chosen Cacopsylla coccinea as the research object，conducted the determination of its mitochondrial（mt）genome，and analyzed its sequence characteristics. With the technology of PCR and gene cloning，the present study proved that the complete mitochondrial genome（MG）of C. coccineae was 14 832 bp of a full length. It encoded 37 genes，including 13 protein-encoding genes，22 transferred RNA genes，2 ribosomal RNA genes，and 1 non-coding region with high AT content. Using 3 insects of C. coccineae，Paratrioza sinica and Pachypsylla venusta as study material，we analyzed the molecular composition features of Psyllidae mitochondrial genome with comparative genomics and bioinformatics，and the results were as follows：（1）the sizes of MG from 3 insects were similar；（2）trnV gene in C. coccineae rearranged while compared with the other 2；（3）the interval in P. venusta was the longest，and the overlap area in P. sinica was the largest；（4）AT contents in 3 insects were similar，with about 70%；（5）Base mismatch in 3 insects existed，with the highest AT content of 3rd -site codon in H-strand and L-strand；and （6）the base substitution rate of nad 5，nad6 and cob genes in MG was < 1，indicating that they were under negative selection.

Key words: Cacopsylla coccinea, mitochondrial genome, sequence analysis

JIN Qiao, LIU Xia, GAN Zhi-Kai. Sequencing and Analyzing Mitochondrial Genome of Cacopsylla coccinea[J]. Biotechnology Bulletin, 2018, 34(11): 127-135.

References

[1] Weintraub PG, Beanland LA.Insect vectors of phytoplasmas[J]. Annu Rev Entomol, 2006, 51:91-111.
[2] 张晓蓉, 陈义光. 湘西地区木通属植物调查[J]. 吉首大学学报:自然科学版, 2001, 22(2):38-40.
[3] Martinez AL, Wallace JM.Citrus leaf mottle-yellows disease in the Philippines and transmission of the causal virus by a psyllid, Diaphorina citri[J]. Plant Dis Rep, 1967, 51(8):692-695.
[4] Yang XB, Zhang YM, Hua L, et al.Life history and life tables of Bactericera cockerelli(Hemiptera:Psyllidae)on potato under laboratory and field conditions in the lower Rio Grande Valley of Texas[J]. J Econ Entomol, 2010, 103(5):1729-1734.
[5] Hodkinson ID.The biology of the Psylloidea(Homoptera):a review[J]. Bulletin of Entomological Research, 1974, 64(2):325-338.
[6] Tsai JH, Liu YH.Biology of Diaphorina citri(Homoptera:Psyllidae)on four host plants[J]. J Econ Entomol, 2000, 93(6):1721-1725.
[7] Liu D, Trumble J T.Comparative fitness of invasive and native populations of the potato psyllid(Bactericera cockerelli)[J]. Entomologia Experimentalis et Applicata, 2007, 123(1):35-42.
[8] Sun J R, Li Y, Yan S, et al.Microsatellite marker analysis of genetic diversity of Cacopsylla chinensis(Yang et Li)(Hemiptera:Psyllidae)populations in China[J]. Acta Entomol Sin, 2011, 54:820-827.
[9] 陈慈相, 张倩, 谢金招, 等. 赣南地区柑桔黄龙病发生规律研究[J]. 中国南方果树, 2015, 44(6):43-45.
[10] 黄建昌, 梁关生, 刘小冬, 等. 几种杀虫剂对柑橘木虱的防治试验初报[J]. 中国园艺文摘, 2018, 1:47-48.
[11] 唐毅. 西南地区木虱总科区系分类研究(半翅目:胸喙亚目)[D]. 贵阳:贵州大学, 2007.
[12] 魏书军, 陈学新. 昆虫比较线粒体基因组学研究进展[J]. 应用昆虫学报, 2012, 48(6):1573-1585.
[13] 于卫卫. 螺旋粉虱种群分化及线粒体全基因组和核糖体基因序列分析[D]. 扬州:扬州大学, 2012.
[14] 李天杰, 曹延祥, 赵红翠, 等. 动物线粒体基因组测序方法的研究进展[J]. 天津医药, 2016(6):796-800.
[15] Saccone C, Pesole G.Handbook of comparative genomics:principles and methodology[J]. Handbook of Comparative Genomics Principles & Methodology, 2003(4):366-368.
[16] Thao MLL, Baumann L, Baumann P.Organization of the mitochondrial genomes of whiteflies, aphids, and psyllids(Hemiptera, Sternorrhyncha)[J]. BMC Evolutionary Biology, 2004, 4(30):25-25.
[17] Nishibori M, Tsudzuki M, Hayashi T, et al.Complete nucleotide sequence of the Coturnix chinensis(Blue-breasted quail)mitochondorial genome and a phylogenetic analysis with related species[J]. Journal of Heredity, 2002, 93(6):439-444
[18] Yamauchi MM, Miya MU, Nishida M.Complete mitochondrial DNA sequence of the swimming crab, Portunus trituberculatus(Crustacea:Decapoda:Brachyura)[J]. Gene, 2003, 311:129-135.
[19] Simon C, Frati F, Beckenbach A, et al.Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers[J]. Ann Entomol Soc Am, 1994, 87(6):651-701.
[20] Saccone C, Pesole G.Handbook of comparative genomics:principles and methodology.[J]. Handbook of Comparative Genomics Principles & Methodology, 2003(4):366-368.
[21] Thao MLL, Baumann L, Baumann P.Organization of the mitochondrial genomes of whiteflies, aphids, and psyllids(Hemiptera, Sternorrhyncha)[J]. BMC Evolutionary Biology, 2004, 4(30):25-25.
[22] Yamauchi MM, Miya MU, Nishida M.Complete mitochondrial DNA sequence of the swimming crab, Portunus trituberculatus(Crustacea:Decapoda:Brachyura)[J]. Gene, 2003, 311:129-135.
[23] Zhang DX, Hewitt GM.Assessment of the universality and utility of a set of conserved mitochondrial cox1 primers in insects[J]. Insect Molecular Biology, 1997, 6(2):143-150.