Control Effects of 56 Extracts of Chinese Traditional Medicine on Cotton Wilt Disease

doi:10.13560/j.cnki.biotech.bull.1985.2018-0025

Abstract

Abstract:

The bacteriostatic activity and control effects of 56 extracts from Chinese traditional medicine were screened and determined in order to acquire the ingredients that may effectively suppress the mycelial growth of Verticillium dahlia and increase the control effects on cotton wilt disease. The results of growth rate showed that the inhibition rate on V. dahlia mycelial growth by 26 of all tested 56 extracts was up to 50%,which were mainly distributed in 9 medicinal plants of Schisandra chinensis（Turcz.）baill,Schisandra propinqua var. sinensis,Manglietiastrum sinicum,Euphorbia sikkimensics,etc. Further,the cotton plants were treated by the extracts and the statistics of disease index and control effect was conducted. The results explained that the chloroform：acetone（3∶2,V/V）extract of S. chinensis（sampled from Heilongjiang province）presented significant control effects to cotton wilt disease. The stem dissection revealed that the extract effectively suppressed the browning of vascular bundle caused by wilt disease,and the symptom of leaf-falling from wilt disease in whole plant was significantly alleviated. The above data indicate that the chloroform：acetone（3∶2,V/V）extract from S. chinensis not only effectively inhibits the growth of V. dahlia in vitro,but also induces the resistance in cotton,thus decreases the browning of vascular bundle and alleviates the occurrence of cotton wilt disease..

Key words: Chinese traditional medicine, Verticillium dahliae, cotton wilt disease, induced resistance

JIANG Lan, PANG Jin-huan, XIAO Wei-lie, ZHANG Guo-li, LIU Jun, YANG Chao. Control Effects of 56 Extracts of Chinese Traditional Medicine on Cotton Wilt Disease[J]. Biotechnology Bulletin, 2018, 34(2): 128-134.

References

[1] 林玲, 张昕, 邓晟. 棉花黄萎病研究进展[J]. 棉花学报, 2014, 26(3):260-267.
[2] 李成伟, 丁锦平, 刘冬梅, 等. 棉花黄萎病及抗病育种研究进展[J]. 棉花学报, 2008, 20(5):385-390.
[3] 石磊, 侯振安, 尹飞虎. 生物农药在棉花黄萎病防治上的研究与应用[J]. 新疆农垦科技, 2017, 40(4):25-28.
[4] 朱荷琴, 冯自力, 宋晓轩, 刘雪英. 22种中药提取物对棉花黄萎病菌的抑菌活性[J]. 棉花学报, 2007, 19(6):489-492.
[5] 孟庆华. 一种防治棉花黄萎病的复方中草药制剂及其生产方法[P]. 中国专利:ZL201310045556. 8, 2014-3-5.
[6] 王美入, 姚琴, 杨家荣, 等. 5种植物提取物及残体对棉花黄萎病菌的抑制作用. 西北农林科技大学学报:自然科学版, 2014, 42(4):137-142.
[7] 王庆芳, 叶华智. 几种化学物质对茄子抗黄萎病的诱导作用[J]. 中国蔬菜, 2008, 1(9):24-27.
[8] 朱荷琴, 宋晓轩, 简桂良. 棉花黄萎病菌致病力变异生理机制的初步研究[J]. 棉花学报, 2004, 16(5):275-279.
[9] 曹静, 客邵英, 王树桐, 等. 15种中草药提取物对马铃薯晚疫病的抑制效果[J]. 江苏农业科学, 2006, (4):51-52.
[10] 孔秋莲, 张明融, 章丽丽. 中草药提取物对两种果蔬致腐真菌的抑制作用[J]. 保鲜与加工, 2002, 2(3):17-18.
[11] 尚善斋. 两种五味子科和两种木兰科植物的化学成分及其生物活性研究[D]. 北京:中国科学院大学, 2013.
[12] 范君文, 于录, 马兰芝, 等. 29种中药提取物抗结核药物敏感性研究[J]. 中国农学通报, 2009, 25(24):1-7.
[13] 杨大松, 魏建国, 杨永平, 等. 黄苞大戟化学成分研究[J]. 中国中药杂志. 2013, 38(23):4094-4098.
[14] 肖瑞颖, 王伟, 钱俊峰. 五味子不同产地、部位及提取工艺对木脂素成分含量影响的研究[J]. 天津药学, 2016, 28(5):8-11.
[15] Frappe EF, Thomma BPHJ.Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum[J]. Molecular Plant Pathology, 2006, 7(2):71-86.
[16] 赵风轩, 戴小枫. 棉花黄萎病菌的侵染过程[J]. 基因组学与应用生物学, 2009, 28(4):786-792.
[17] Paplomatas EJ, Bassett DM, et al.Incidence of Verticillium wilt and yield losses of cotton cultivars based on soil inoculum density[J]. Phytopathology, 1992, 82(12):1417-1420.
[18] Pullman GS, Devay JE.Effect of soil flooding and paddy rice culture on the survival of Verticillium dabliae and incidence of Verticillium wilt in cotton[J]. Phytopathology, 1981, 71(12):1285-1289.
[19] 谢仲权, 牛树琦. 天然植物饲料添加剂[M]. 北京:中国农业科学与技术出版社, 2004.
[20] 乔金玲, 胡永金. 植物源提取物抑菌机理及其应用研究进展[J]. 天然产物研究与开发, 2010, 22:275-279.