Biotechnology Bulletin ›› 2018, Vol. 34 ›› Issue (3): 67-74.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0742

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Comparison and Optimization of the Detection Method for Pseudomonas aeruginosa

ZHANG Fan1，2, LI Shu-yao2, ZHANG Zi-hao1, CAI Xue-feng2, REN Yan2, LIU Kai2, HOU Cui-yan2, YI Xin-xin1, GAO Xiu-zhi1   

  1. 1. Faculty of Food Science and Engineering，Beijing University of Agriculture，Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue，Beijing Laboratory of Food Quality and Safety，Beijing Engineering Laboratory of Probiotics Key Technology Development，Beijing 102206;
    2. China National Food Safety Supervision and Inspection Center，Beijing 100094
  • Received:2017-09-07 Online:2018-03-20 Published:2018-04-10

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Abstract: This work aimed to obtain a simple-operation，more accurate method and requiring low test condition by analyzing and comparing 3 Pseudomonas aeruginosa detection methods. The identification results of VITEK 2 Compact automatic bacterial identification system were used as reference，the test methods of P. aeruginosa in GB 8538.57-2016 and GB/T7918.4-1987 were applied to identify 27 strains. The results showed that the accuracy of the GB 8538.57-2016 method was the lowest，and the false positive rate was 29.6%，while the false positive rate of GB/T7918.4-1987 was 7.4%. In the case of GB 8538.57-2016 P. aeruginosa as an example，three steps were added to the optimized method，the blood agar plate culture，Gram stain and microscopy，and pyocyanin test for all suspicious colonies of the blood agar plate. The 27 test strains were verified by the optimized detection method，and the results were consistent with those of VITEK 2 Compact.

Key words: Pseudomonas aeruginosa, mineral water, VITEK 2 Compact, GB 8538.57-2016, GB/T7918.4-1987