Activity Variations of Protective Enzymes in Paracoccus marginatus After Fed Different Cassava Cultivars

doi:10.13560/j.cnki.biotech.bull.1985.2018-0054

Abstract

Abstract: To explore the mechanism of Paracoccus marginatus selecting host plants，the activity variations of peroxidase（POD），polyphenol oxidase（PPO），catalase（CAT）and superoxide dismutase（SOD）in P. marginatus after fed different cassava cultivars for 48 h were determined by ultraviolet spectrophotometry. The results showed that the enzymes’ activities in adult females fed with cultivar C1115，Swiss F21 and Myanmar were significantly lower than those fed with the control cultivar BRA900（P ﹤0.05），the decreasing range were listed as：41%-43%（POD），60%-63%（PPO），35%-55%（SOD）and 54%-60%（CAT），while those in adult females fed with cultivar SC205 and ZM9066 did not show significant difference. Above results indicate that cassava cultivar C1115，F21 and Myanmar show strong inhibitory effects on the activities of POD，PPO，SOD and CAT in P. marginatus，while BRA900，SC205 and ZM9066 do not show significant inhibitory effects on those protective enzymes，which inclines them to be damaged by P. marginatus.

Key words: Paracoccus marginatus, cassava cultivar, protective enzymes, activity variation

WANG Ya-ru ,LIANG Xiao ,WU Chun-ling ,CHEN Qing ,ZHAO Hui-ping. Activity Variations of Protective Enzymes in Paracoccus marginatus After Fed Different Cassava Cultivars[J]. Biotechnology Bulletin, 2018, 34(6): 115-119.

References

[1] Saengyot S, Burikam I. Host plants and natural enemies of papaya mealybug, Paracoccus marginatus（Hemiptera：Pseudococcidae）in Thailand[J] . Thai Journal of Agricultural Science, 2011, 44（3）：197-205.
[2] Galanihe LD, Jayasundera MUP, Vithana A, et al. Occurrence, distribution and control of papaya mealybug, Paracoccus marginatus（Hemiptera：Pseudococcidae）, an invasive alien pest in Sri Lanka[J] . Tropical Agricultural Research & Extension, 2011, 13（3）：81-86.
[3] Mastoi MI, Azura AN, Muhamad R, et al. Survey of papaya mealybug, Paracoccus marginatus（Hemiptera：Pseudococcidae）and its natural enemies in Penninsular Malaysia[J] . Pakistan Journal of Agriculture Agricultural Engineering Veterinary Sciences, 2014, 21（6）：72-78.
[4] 卢辉, 卢芙萍, 梁晓, 等. 木瓜秀粉蚧在海南的适生性及空间分布型研究[J] . 热带作物学报, 2016, 37（10）：62-68.
[5] Lu F, Chen Q, Chen Z, et al. Effects of heat stress on development, reproduction and activities of protective enzymes in Mononychellus mcgregori[J] . Exp Appl Acarol, 2014, 63（2）：267-284.
[6] Lu F, Liang X, Lu H, et al. Overproduction of superoxide dismutase and catalase confers cassava resistance to Tetranychus cinnabarinus[J] . Scientific Reports, 2017, 7（40179）.
[7] 陈青, 张银东. 3种氧化酶与辣椒抗蚜性的相关性[J] . 热带作物学报, 2004, 25（3）：42-46.
[8] 徐雪莲, 张银东, 符悦冠, 等. 蚜虫侵害对西瓜保护酶活性的影响[J] . 北方园艺, 2014, 12（1）：109-111.
[9] Yang ZW, Duan XN, Jin S, et al. Regurgitant derived from the tea geometrid Ectropis obliqua suppresses wound-induced polyphenol oxidases activity in tea plants[J] . Journal of Chemical Ecology, 2013, 39（6）：744-752.
[10] Liang X, Chen Q, Lu H, et al. Increased activities of peroxidase and polyphenol oxidase enhance cassava resistance to Tetranychus urticae[J] . Exp Appl Acarol, 2017, 71（3）：195-203.
[11] Lu F, Chen Z, Lu H, et al. Effects of resistant and susceptible rubber germplasms on development, reproduction and protective enzyme activities of Eotetranychus sexmaculatus（Acari：Tetranychidae）[J] . Exp Appl Acarol, 2016, 69（4）：427-433.
[12] 陈青. 几种生化物质与辣椒抗蚜性的相关性[J] . 园艺学报, 2002, 29（6）：533-536.
[13] 王琦, 董合忠. 棉花次生代谢物质及其抗虫活性研究进展[J] . 棉花学报, 2013, 25（6）：557-563.
[14] 郭广君, 孙帅, 王孝宣, 等. 番茄抗B型烟粉虱相关叶表腺毛性状及其次生代谢物质分析[J] . 园艺学报, 2016, 43（8）：1493-1503.
[15] 刘佳妮, 黄鹤平, 华金珠, 等. 烟碱对马铃薯块茎蛾幼虫保护酶和解毒酶的影响[J] . 贵州农业科学, 2015, 2（3）：78-81.
[16] 张秀丽, 王炎, 徐东, 等. 桑叶提取物1-DNJ对甜菜夜蛾幼虫生长和保护酶活性的影响[J] . 中国农学通报, 2014, 30（22）：316-320.
[17] Apel K, Hirt H. Reactive oxygen species：metabolism, oxidative stress, and signal transduction[J] . Annual Review of Plant Biol, 2004, 55（4）：373-382.
[18] Mittler R. Oxidative stress, antioxidants and stress tolerance[J] . Trends in Plant Science, 2002, 7（9）：405-410.
[19] Heng-Moss T, Sarath G, Baxendale F, et al. Characterization of oxidative enzyme changes in buffalograsses challenged by Blissus occiduus[J] . J Econ Entomol, 2004, 97（3）：1086-1095.
[20] Barbehenn RV. Gut-based antioxidant enzymes in a polyphagous and a graminivorous grasshopper[J] . Journal of Chemical Ecology, 2002, 28（7）：1329-1335.
[21] Aucoin RR, Philogène BJR, Arnason JT. Antioxidant enzymes as biochemical defenses against phototoxin-induced oxidative stress in three species of herbivorous lepidoptera[J] . Archives of Insect Biochemistry & Physiology, 1991, 16（2）：139-152.
[22] 吴丰年, 岑伊静, 梁广文. 不同离体寄主及其成熟度对亚洲柑桔木虱存活的影响[J] . 应用昆虫学报, 2015（3）：679-685.