Biotechnology Bulletin ›› 2019, Vol. 35 ›› Issue (7): 70-75.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0109

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Identification of Bacillus cereus Strain Producing Fibrinolytic Enzyme from Sipunculus nudus

LI Xiao-mei, ZHOU Zong-hui, YIN Xiu-hua, JIANG Hong-rui, LIU Xiao-ling   

  1. Institute of Light Industry and Food Engineering,Guangxi University,Nanning 530004
  • Received:2019-01-31 Online:2019-07-26 Published:2019-07-29

Abstract: Screening and isolating the fibrinolytic strains of Sipunculus nudus in the North of Guangxi by a combination of skim milk plate and fibrin plate assay,the specificity and fibrinolytic mechanism of the strain were analyzed. As a result,5 strains isolated and screened from Sipunculus nudus showed ability of fibrin degradation. The crude enzyme fermented from the strain GXUSP-1 presented the maximal fibrinolytic activity as 303.2 U/mL(equivalent to urinary kinase activity unit). GXUSP-1 was identified as Bacillus cereus,and belonged to marine symbiotic bacteria. Meanwhile,thrombolytic effect in vitro of the crude enzyme fermented from GXUSP-1 was significant,which dissolved thrombus both directly and indirectly. GXUSP-1 demonstrates promising potential industrial application.

Key words: Sipunculus nudus, fibrinolytic enzyme, Bacillus cereus, identification