Research Progresses on the Synthetic Biology of Terpenes in Saccharomyces cerevisiae

doi:10.13560/j.cnki.biotech.bull.1985.2020-0565

Abstract

Abstract:

Terpenoids are widely used in the pharmaceutical,food,and cosmetic industries due to their unique and diverse biological activities. Saccharomyces cerevisiae,the simplest eukaryotic cell,is the preferred cell factory for complex terpenoids biosynthesis with high suitability of enzymes from eukaryotic sources. In recent years,construction and optimization of terpenoid metabolic pathways in S. cerevisiae have received much attention in synthetic biological researches. Here we review the synthetic biological strategies of high-value terpenoids from four aspects：optimizing isoprene precursor pathway enzymes,reforming central carbon metabolism,balancing competitive pathways,and expressing heterologous cytochrome P450 enzymes,aiming at providing references for the further researches of terpenoids biosynthesis in S. cerevisiae.

Key words: terpenoids, biosynthesis, synthetic biology, Saccharomyces cerevisiae

LI Jia-xiu, CAI Qian-ru, WU Jie-qun. Research Progresses on the Synthetic Biology of Terpenes in Saccharomyces cerevisiae[J]. Biotechnology Bulletin, 2020, 36(12): 199-207.

Figures/Tables 4

References 65

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