Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (12): 82-90.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0375
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LI Wei-na1(), SHEN Dong-ling2, ZHANG Yu-xing1, LIU Xue-tong1, IRBIS Chagan2()
Received:
2020-04-06
Online:
2020-12-26
Published:
2020-12-22
Contact:
IRBIS Chagan
E-mail:liweina502361483@163.com;chaganirbis2019@126.com
LI Wei-na, SHEN Dong-ling, ZHANG Yu-xing, LIU Xue-tong, IRBIS Chagan. Cloning and Enzymatic Identification of Thermo-tolerant and Endotype Alginate Lyase Gene from Mangrovibacterium sp. SH-52[J]. Biotechnology Bulletin, 2020, 36(12): 82-90.
Step | Total protein/(mg·L-1) | Totalactivity/U | Specific activity/(U·mg-1) | Yield/% | Purification(fold) |
---|---|---|---|---|---|
Cell extract | 162.0 | 421.2 | 2.6 | 100.0 | 1 |
GST Sefinose Resin BSP091 | 3.2 | 41.6 | 13.0 | 9.8 | 5 |
Step | Total protein/(mg·L-1) | Totalactivity/U | Specific activity/(U·mg-1) | Yield/% | Purification(fold) |
---|---|---|---|---|---|
Cell extract | 162.0 | 421.2 | 2.6 | 100.0 | 1 |
GST Sefinose Resin BSP091 | 3.2 | 41.6 | 13.0 | 9.8 | 5 |
Reagent | Concentration/(mmol·L-1) | Relative activity/% |
---|---|---|
None | 0 | 100 |
NaCl | 1 | 101 |
50 | 156 | |
100 | 158 | |
KCl | 1 | 115 |
MgCl2 | 1 | 112 |
NiCl2 | 1 | 7 |
ZnCl2 | 1 | 114 |
CoCl2 | 1 | 92 |
HgCl2 | 1 | 8.5 |
CaCl2 | 1 | 104 |
MnCl2 | 1 | 85.1 |
EDTA | 1 | 33.5 |
Reagent | Concentration/(mmol·L-1) | Relative activity/% |
---|---|---|
None | 0 | 100 |
NaCl | 1 | 101 |
50 | 156 | |
100 | 158 | |
KCl | 1 | 115 |
MgCl2 | 1 | 112 |
NiCl2 | 1 | 7 |
ZnCl2 | 1 | 114 |
CoCl2 | 1 | 92 |
HgCl2 | 1 | 8.5 |
CaCl2 | 1 | 104 |
MnCl2 | 1 | 85.1 |
EDTA | 1 | 33.5 |
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