Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (5): 145-149.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0827

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Polymorphism of OLR1 Gene and Its Association with Meat Quality Traits in Sujiang Pigs

REN Shan-mao, WANG Wen-hui, TAO Yong   

  1. Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300
  • Received:2019-09-06 Online:2020-05-26 Published:2020-06-03

Abstract: This work is to investigate the polymorphism of lipoprotein-receptor-l(OLR1)gene and its association with the meat quality traits in Sujiang pigs. The Pst genetic polymorphism of OLR1 gene in Sujiang pigs was detected by PCR-RFLP,and the association between OLR1 genotype and meat quality traits was analyzed with one-way ANOVA. The results showed that Pst Ⅰ polymorphism in the intron 5 of OLR1 gene in the experimental population of Sujiang pig was found,and three genotypes(CC,CD and DD)were detected. The polymorphism information content was moderate. The difference of the water loss rate and marbling between CC and DD genotypes of OLR1 gene were significant(P<0.05),and the b value of meat color of CD genotype by color difference meter was significantly higher than that of DD genotype(P<0.05). Therefore,the detected PCR-RFLP-Pst I polymorphism of OLR1 gene is significantly correlated with some meat traits such as marbling,and it could be used as a potential candidate gene of meat quality traits in the continuous breeding of Sujiang pigs.

Key words: Sujiang pig, OLR1 gene, PCR-RFLP-Pst Ⅰ, meat quality trait, association analysis