Regulation of Lanthanum on Rice Transcriptome Patterns Under Copper Stress

doi:10.13560/j.cnki.biotech.bull.1985.2020-0110

Abstract

Abstract: This work is to investigate the effects of rare earth lanthanum on rice transcriptome under copper stress,and to identify key genes and functions of lanthanum-regulated responses in rice against copper stress. The optimal copper stress and lanthanum treatment concentrations were screened by antioxidant enzyme activity,transcriptome sequencing and differential expression analysis were performed,and differential expression levels were verified by qRT-PCR. Differential expression analysis of sequencing data revealed that 3 222 genes were significantly up-regulated and 3 798 genes were significantly down-regulated. Four cell wall defense-related genes CHIT13,Laccase,Expansin,and GH3.4 were verified by qRT-PCR. Total 95 Gene Ontology terms and 112 KEGG pathways were obtained by function and pathway enrichment analysis,and they enriched in molecular functions such as kinase and oxygen molecule binding,cell components such as cell wall and plasma membrane,biological processes such as secondary metabolism and lipid metabolism,as well as pathways e.g. phenylpropane biosynthesis,and plant hormone signal transduction. The molecular mechanism is that La increases the tolerance of rice to copper stress by regulating cell wall formation and components.

Key words: rice, transcriptome, copper stress, lanthanum, sequencing

ZHONG Yu-qing, CHEN Jia-jia. Regulation of Lanthanum on Rice Transcriptome Patterns Under Copper Stress[J]. Biotechnology Bulletin, 2020, 36(8): 8-14.

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