Isolation,Screening and Identification of Desulfurization Bacteria from Kitchen Waste

doi:10.13560/j.cnki.biotech.bull.1985.2019-0825

Abstract

Abstract: The objective of this work is to obtain microorganisms that can reduce hydrogen sulfide emission in the kitchen waste compost process. Kitchen waste compost was enriched,screened and identified to preliminarily determine the genera of bacteria,and the optimal culture conditions of desulfurization bacterium for deodorization were determined via single factor experiment. Results showed that the two desulfurization strains were identified as KW6 and KSW8,respectively. KW6 belonged to Bacillus and KSW8 belonged to Rhizobium. The remove rate of hydrogen sulfide by KW6 was the highest,when the culture temperature was 30℃,the pH value was 7.5 and the inoculation was 12%,which was 83.5%,65.3% and 73.8%,respectively. The removal rate of hydrogen sulfide by KSW8 was the highest,when the culture temperature was 35℃,the pH value was 6.5 and the inoculation was 20%,which was 85.8%,75.8% and 72.4%,respectively. Comparing with CK,KW6 and KSW8 reduced the releasing amount of hydrogen sulfide by 28.83% and 50.08%,respectively. The optimal deodorization culture conditions for KW6 were temperature 30℃,pH 7.5 and inoculation 12%.The optimal deodorization culture conditions for KSW8 were 35℃,pH6.5 and inoculation 20%.

Key words: kitchen waste, desulfurization bacterium, screening, appraisal, conditions optimization

WANG Xiao-li, ZHANG Wen-wen, WU Qing, CAO Yun-e. Isolation,Screening and Identification of Desulfurization Bacteria from Kitchen Waste[J]. Biotechnology Bulletin, 2020, 36(8): 87-95.

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