Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (11): 125-133.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1011
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GUO Yu-feng(), WANG Qiao, WANG Xiang-feng, ZHOU Ting, YANG Xiao-jun, WANG Qing-ji, WANG Li()
Received:
2021-08-12
Online:
2021-11-26
Published:
2021-12-03
Contact:
WANG Li
E-mail:yufeng_12021@163.com;haoyou0102@163.com
GUO Yu-feng, WANG Qiao, WANG Xiang-feng, ZHOU Ting, YANG Xiao-jun, WANG Qing-ji, WANG Li. Screening of Superior Strains Isolated from Wild Cordyceps militaris in Mountain Tai[J]. Biotechnology Bulletin, 2021, 37(11): 125-133.
菌株编号Strain | 日均菌丝长速Daily mycelial growth rate/(mm·d-1) | 菌落特征Characteristics of colony |
---|---|---|
1 | 1.47±0.23 cd | 致密、淡黄色、边缘整齐 Dense,light yellow,and neat edge |
2 | 1.76±0.27 abc | 致密、淡黄色、边缘整齐 Dense,light yellow,and neat edge |
3 | 1.25±0.04 d | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
4 | 1.54±0.14 bcd | 致密、淡黄色、边缘整齐 Dense,light yellow,and neat edge |
5 | 1.72±0.16 abc | 致密、淡黄色、边缘不整齐 Dense,light yellow,and irregular edge |
6 | 1.88±0.11 ab | 绒毛状、淡黄色、边缘整齐 Fluffy,light yellow,and neat edge |
7 | 0.83±0.14 e | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
8 | 1.64±0.23 abc | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
9 | 1.69±0.02 abc | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
10 | 1.93±0.21 a | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
11 | 1.88±0.11 ab | 致密、淡黄色、边缘整齐 Dense,light yellow,and neat edge |
12 | 0.68±0.13 e | 绒毛状、白色、边缘发散 Dense,light yellow,and edge divergence |
13 | 1.44±0.24 cd | 致密、淡黄色、环纹状 Dense,light yellow,and ring shape |
14 | 1.58±0.25 abcd | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
Table 1 Mycelia growth rate and the colony morphology
菌株编号Strain | 日均菌丝长速Daily mycelial growth rate/(mm·d-1) | 菌落特征Characteristics of colony |
---|---|---|
1 | 1.47±0.23 cd | 致密、淡黄色、边缘整齐 Dense,light yellow,and neat edge |
2 | 1.76±0.27 abc | 致密、淡黄色、边缘整齐 Dense,light yellow,and neat edge |
3 | 1.25±0.04 d | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
4 | 1.54±0.14 bcd | 致密、淡黄色、边缘整齐 Dense,light yellow,and neat edge |
5 | 1.72±0.16 abc | 致密、淡黄色、边缘不整齐 Dense,light yellow,and irregular edge |
6 | 1.88±0.11 ab | 绒毛状、淡黄色、边缘整齐 Fluffy,light yellow,and neat edge |
7 | 0.83±0.14 e | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
8 | 1.64±0.23 abc | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
9 | 1.69±0.02 abc | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
10 | 1.93±0.21 a | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
11 | 1.88±0.11 ab | 致密、淡黄色、边缘整齐 Dense,light yellow,and neat edge |
12 | 0.68±0.13 e | 绒毛状、白色、边缘发散 Dense,light yellow,and edge divergence |
13 | 1.44±0.24 cd | 致密、淡黄色、环纹状 Dense,light yellow,and ring shape |
14 | 1.58±0.25 abcd | 绒毛状、白色、边缘整齐 Fluffy,white,and neat edge |
菌株编号 Strain | 转色情况 Color-converting situation | 成熟周期 Maturating period/d | 子实体鲜重 Fresh weight of fruit body/g | 子实体干重 Dry weight of fruit body/g | 生物学效率 Biological efficiency/% | 子实体形态 Fruit body morphology |
---|---|---|---|---|---|---|
1 | ++ | 54.3±1.2 a | 18.67±3.45 bcd | 3.91±0.39 abc | 46.67±0.09 bcd | 橘黄粗短圆头 |
2 | +++ | 46.3±2.1 c | 22.12±4.12 abc | 4.95±0.88 ab | 55.31±0.10 abc | 橙黄粗长圆头 |
3 | + | 51.3±1.2 b | 23.19±3.11ab | 4.44±0.49 ab | 57.80±0.78 ab | 淡黄粗短尖头 |
4 | +++ | 46.3±1.5c | 23.23±0.78 ab | 5.15±0.17 a | 58.08±0.02 ab | 淡黄细长尖头 |
5 | ++ | 55.3±2.5 a | 24.23±2.85 a | 5.18±0.32 a | 60.58±0.71 a | 橙黄粗短圆头 |
6 | ++ | 56.7±1.5 a | 17.62±0.61 cd | 3.62±0.12 bcd | 44.06±0.15 cd | 淡黄细短尖头 |
9 | + | 56.3±1.5 a | 15.54±1.24 d | 2.66±0.81 cd | 38.85±0.31 d | 淡黄细短圆头 |
11 | ++ | 51.3±1.5 b | 20.71±2.10 abcd | 4.35±0.93 ab | 51.77±0.05 abcd | 淡黄细短尖头 |
13 | + | 43.3±1.5 d | 16.59±4.07 d | 4.11±1.20 d | 41.46±0.10 d | 淡黄粗短尖头 |
Table 2 Comparison of yield and cultivation period of 9 strains C. militaris
菌株编号 Strain | 转色情况 Color-converting situation | 成熟周期 Maturating period/d | 子实体鲜重 Fresh weight of fruit body/g | 子实体干重 Dry weight of fruit body/g | 生物学效率 Biological efficiency/% | 子实体形态 Fruit body morphology |
---|---|---|---|---|---|---|
1 | ++ | 54.3±1.2 a | 18.67±3.45 bcd | 3.91±0.39 abc | 46.67±0.09 bcd | 橘黄粗短圆头 |
2 | +++ | 46.3±2.1 c | 22.12±4.12 abc | 4.95±0.88 ab | 55.31±0.10 abc | 橙黄粗长圆头 |
3 | + | 51.3±1.2 b | 23.19±3.11ab | 4.44±0.49 ab | 57.80±0.78 ab | 淡黄粗短尖头 |
4 | +++ | 46.3±1.5c | 23.23±0.78 ab | 5.15±0.17 a | 58.08±0.02 ab | 淡黄细长尖头 |
5 | ++ | 55.3±2.5 a | 24.23±2.85 a | 5.18±0.32 a | 60.58±0.71 a | 橙黄粗短圆头 |
6 | ++ | 56.7±1.5 a | 17.62±0.61 cd | 3.62±0.12 bcd | 44.06±0.15 cd | 淡黄细短尖头 |
9 | + | 56.3±1.5 a | 15.54±1.24 d | 2.66±0.81 cd | 38.85±0.31 d | 淡黄细短圆头 |
11 | ++ | 51.3±1.5 b | 20.71±2.10 abcd | 4.35±0.93 ab | 51.77±0.05 abcd | 淡黄细短尖头 |
13 | + | 43.3±1.5 d | 16.59±4.07 d | 4.11±1.20 d | 41.46±0.10 d | 淡黄粗短尖头 |
活性物质Active substance/(mg·g-1) | 菌株Strain | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 9 | 11 | 13 | ||
虫草素 Cordycepin | 3.87±0.22 f | 5.43±0.30 e | 6.13±0.61 d | 7.13±0.09 c | 2.51±0.23 g | 2.73±0.07 g | 0.80±0.05 h | 9.03±0.19 b | 15.14± 0.16 a | |
N6-(2-羟乙基)腺 苷Adenosine | 0.42±0.02 d | 0.38±0.01 e | 0.59±0.01 b | 0.56±0.01 c | 0.25±0.01 g | 0.31±0.01 f | 0.26±0.02 g | 0.31±0.02 f | 0.66±0.01 a | |
多糖 Polysac-charides | 43.97±3.86 d | 90.44±3.68 a | 74.58±0.98 b | 84.68±0.12 a | 90.04±1.34 a | 61.90±1.51 c | 75.46±4.57 b | 72.82±6.42 b | 43.27± 3.49 d | |
β-类胡萝卜素 β-Carotene | 0.065±0.002 a | 0.055±0.001 b | 0.036±0.001 e | 0.036±0.001 e | 0.034±0.001 f | 0.036±0.001 e | 0.047±0.001 c | 0.032±0.002 f | 0.041± 0.001 d | |
叶黄素 Xanthophyll | 0.17±0.01 e | 0.32±0.01 b | 0.14±0.01 fg | 0.13±0.01 g | 0.19±0.01 d | 0.13±0.01 g | 0.654±0.01 a | 0.233±0.01 c | 0.15±0.01 f |
Table 3 Contents of different active substances in the fruiting bodies of 9 C. militaris strains
活性物质Active substance/(mg·g-1) | 菌株Strain | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 9 | 11 | 13 | ||
虫草素 Cordycepin | 3.87±0.22 f | 5.43±0.30 e | 6.13±0.61 d | 7.13±0.09 c | 2.51±0.23 g | 2.73±0.07 g | 0.80±0.05 h | 9.03±0.19 b | 15.14± 0.16 a | |
N6-(2-羟乙基)腺 苷Adenosine | 0.42±0.02 d | 0.38±0.01 e | 0.59±0.01 b | 0.56±0.01 c | 0.25±0.01 g | 0.31±0.01 f | 0.26±0.02 g | 0.31±0.02 f | 0.66±0.01 a | |
多糖 Polysac-charides | 43.97±3.86 d | 90.44±3.68 a | 74.58±0.98 b | 84.68±0.12 a | 90.04±1.34 a | 61.90±1.51 c | 75.46±4.57 b | 72.82±6.42 b | 43.27± 3.49 d | |
β-类胡萝卜素 β-Carotene | 0.065±0.002 a | 0.055±0.001 b | 0.036±0.001 e | 0.036±0.001 e | 0.034±0.001 f | 0.036±0.001 e | 0.047±0.001 c | 0.032±0.002 f | 0.041± 0.001 d | |
叶黄素 Xanthophyll | 0.17±0.01 e | 0.32±0.01 b | 0.14±0.01 fg | 0.13±0.01 g | 0.19±0.01 d | 0.13±0.01 g | 0.654±0.01 a | 0.233±0.01 c | 0.15±0.01 f |
菌株 Strain | 虫草素 Cordycepin | 腺苷 Adenosine | 多糖 Polysaccharides | β-类胡萝卜素 β-Carotene | 叶黄素 Xanthophyll | 子实体产量 Fruiting body yield | 综合分值(Y) Comprehensive score |
---|---|---|---|---|---|---|---|
1 | 0.21 | 0.41 | 0.01 | 1.00 | 0.08 | 0.36 | 0.24 |
2 | 0.32 | 0.32 | 1.00 | 0.70 | 0.36 | 0.76 | 0.64 |
3 | 0.37 | 0.83 | 0.66 | 0.12 | 0.02 | 0.88 | 0.62 |
4 | 0.44 | 0.76 | 0.88 | 0.12 | 0.00 | 0.88 | 0.69 |
5 | 0.12 | 0 | 0.99 | 0.06 | 0.11 | 1.00 | 0.59 |
6 | 0.13 | 0.15 | 0.39 | 0.12 | 0 | 0.24 | 0.23 |
9 | 0 | 0.02 | 0.68 | 0.45 | 1.00 | 0 | 0.24 |
11 | 0.57 | 0.15 | 0.63 | 0 | 0.20 | 0.59 | 0.52 |
13 | 1.00 | 1.00 | 0 | 0.27 | 0.04 | 0.12 | 0.42 |
Table 4 Scores of the active substances and fruiting body yield of each strain of C. militaris(Unit: Score)
菌株 Strain | 虫草素 Cordycepin | 腺苷 Adenosine | 多糖 Polysaccharides | β-类胡萝卜素 β-Carotene | 叶黄素 Xanthophyll | 子实体产量 Fruiting body yield | 综合分值(Y) Comprehensive score |
---|---|---|---|---|---|---|---|
1 | 0.21 | 0.41 | 0.01 | 1.00 | 0.08 | 0.36 | 0.24 |
2 | 0.32 | 0.32 | 1.00 | 0.70 | 0.36 | 0.76 | 0.64 |
3 | 0.37 | 0.83 | 0.66 | 0.12 | 0.02 | 0.88 | 0.62 |
4 | 0.44 | 0.76 | 0.88 | 0.12 | 0.00 | 0.88 | 0.69 |
5 | 0.12 | 0 | 0.99 | 0.06 | 0.11 | 1.00 | 0.59 |
6 | 0.13 | 0.15 | 0.39 | 0.12 | 0 | 0.24 | 0.23 |
9 | 0 | 0.02 | 0.68 | 0.45 | 1.00 | 0 | 0.24 |
11 | 0.57 | 0.15 | 0.63 | 0 | 0.20 | 0.59 | 0.52 |
13 | 1.00 | 1.00 | 0 | 0.27 | 0.04 | 0.12 | 0.42 |
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